Effect of beta-cyclodextrin on production of L-phenylacetyl carbinol by immobilized cells of Saccharomyces cerevisiae

The rate and extent of microbial transformation of higher concentrations of benzaldehyde substrate to L-phenylacetyl carbinol (L-PAC) by immobilized cells of Saccharomyces cerevisiae ATCC 834 was markedly stimulated by addition of different concentrations of beta-cyclodextrin (BCD) to the fermentati...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1990-07, Vol.36 (3), p.256-262
Main Authors: Mahmoud, W.M. (Zagazig University, Zagazig, Egypt), El-Sayed, A.H.M.M, Coughlin, R.W
Format: Article
Language:English
Subjects:
ACIDE ORGANIQUE
ACIDOS ORGANICOS
ALDEHIDOS
ALDEHYDE
beta -cyclodextrin
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
DEXTRINAS
DEXTRINE
FERMENTACION
FERMENTATION
Fundamental and applied biological sciences. Psychology
IMMOBILISATION
immobilized cells
INMOVILIZACION
L-phenylacetyl carbinol
Methods. Procedures. Technologies
SACCHAROMYCES CEREVISIAE
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Summary:The rate and extent of microbial transformation of higher concentrations of benzaldehyde substrate to L-phenylacetyl carbinol (L-PAC) by immobilized cells of Saccharomyces cerevisiae ATCC 834 was markedly stimulated by addition of different concentrations of beta-cyclodextrin (BCD) to the fermentation medium. With 0.5, 1.0, and 1.5% BCD in the fermentation medium and cumulative doses of benzaldehyde of 12 and 14 g/L, significantly higher yields of L-PAC were obtained, about one- to twofold that of the yields of the control experiments. The favorable effects of BCD were evident in spite of its presence in stoichiometric concentrations significantly lower than those of benzaldehyde. The presence of BCD also appeared to stimulate microbial growth slightly. Enhanced cellular activity was reflected by faster D-glucose consumption and faster benzaldehyde utilization in the presence of BCD
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260360307