Enzymatic activity under tangential flow conditions of photochemically grafted membranes containing immobilized catalase

Catalase has been immobilized within sandwich membranes prepared by the photoinduced grafting of an epoxy‐diacrylate prepolymer onto commercial asymmetric cellulose membranes. The enzymatic activity of the membrane composite of hydrogen peroxide decomposition has been studied in a recirculation appa...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1993-02, Vol.41 (4), p.474-478
Main Authors: Selli, Elena, D'Ambrosio, Andrea, Bellobono, Ignazio Renato
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
catalase
enzymatic membranes
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
photografting
photosynthesized
tangential flow
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Summary:Catalase has been immobilized within sandwich membranes prepared by the photoinduced grafting of an epoxy‐diacrylate prepolymer onto commercial asymmetric cellulose membranes. The enzymatic activity of the membrane composite of hydrogen peroxide decomposition has been studied in a recirculation apparatus under tangential flow conditions without ultrafiltration. The enzymatic membranes were exposed to very low mechanical stresses and showed a very good catalytic performance and durability. Initial reaction rates, measured at 25°C as a function of both substrate concentration and enzyme amount immobilized per unit membrane surface, indicate that the mechanism of action of catalase is not altered after immobilization, although substrate diffusion through the original thin layer of membranes may become rate controlling. © 1993 John Wiley & Sons, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260410411