Detection of microbial contamination in fermentation processes: Mass spectrometric determination of gram-negative bacteria in Leuconostoc mesenteroides cultures

Gas chromatography/mass spectrometry was used to detect the presence of Enterobacter cloacae in cultures of Leuconostoc mesenteroides, an organism used in an industrial process for production of dextrane. The penta‐fluorobenzoyl‐methyl ester derivative of 3‐hydroxy‐myristic acid, a characteristic co...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioengineering 1990-04, Vol.35 (8), p.787-792
Main Authors: Elmroth, Ingrid, Valeur, Anders, Odham, Göran, Larsson, Lennart
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enterobacter cloacae
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Gas chromatography/mass spectrometry was used to detect the presence of Enterobacter cloacae in cultures of Leuconostoc mesenteroides, an organism used in an industrial process for production of dextrane. The penta‐fluorobenzoyl‐methyl ester derivative of 3‐hydroxy‐myristic acid, a characteristic compound of gram‐negative bacteria, was used as the analyte. By using gas chromatography/mass spectrometry with selected ion monitoring, E. cloacae was determined over the range of 1 ppm to 1% in cultures of L. mesenteroides. The proposed analytical approach represents a useful alternative to conventional methods for determining contaminating organisms in industrial fermentation processes.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260350806