High gradient magnetic separation of yeast

High gradient magnetic separation (HGMS) is used to separate nonmagnetic microorganisms from solution by a technique known as seeding. Fine magnetic particles are adhered to the cells' surfaces, making them magnetic and amenable to magnetic separation. Attachment of the sub‐micron, acicular γ‐F...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1991-05, Vol.37 (11), p.1021-1028
Main Authors: Dauer, Richard R., Dunlop, Eric H.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
ferric oxide
Fundamental and applied biological sciences. Psychology
magnetic fields
Methods. Procedures. Technologies
Others
separation techniques
Various methods and equipments
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Summary:High gradient magnetic separation (HGMS) is used to separate nonmagnetic microorganisms from solution by a technique known as seeding. Fine magnetic particles are adhered to the cells' surfaces, making them magnetic and amenable to magnetic separation. Attachment of the sub‐micron, acicular γ‐Fe2O3 seed to the yeast surface occurs irrespective of the solution pH and surface charge and is essentially irreversible. A model is developed to predict the separation of yeast in a high gradient magnetic separator. The effective capture radius is assumed to be proportional to the derived magnetic parameter γ for the case where the dominant competing force to magnetic attraction is the magnetic floc's inertia. Using this parameter, yeast separation in an HGMS unit is predicted. The measured separation of Saccharomyces cerevisiae at differing magnetic seed concentrations and two flow rates supports the above model.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260371106