Fermentation of lactose to ethanol with recombinant yeast in an immobilized yeast membrane bioreactor

A novel concept of membrane bioreactor in which living cells are sandwiched between ultrafiltration (UF) and reverse osmosis (RO) membranes was applied for lactose fermentation to ethanol by genetically engineered yeast cells. The productivity of the Lactophile 13B strains was higher than that of th...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1991-03, Vol.37 (6), p.587-590
Main Authors: Jeong, Y.S. (Rutgers University, Piscataway, NJ), Vieth, W.R, Matsuura, T
Format: Article
Language:English
Subjects:
ADN RECOMBINADO
ADN RECOMBINE
Biological and medical sciences
bioreactors
Biotechnology
ETANOL
ETHANOL
FERMENTACION
FERMENTATION
Fundamental and applied biological sciences. Psychology
GENIE GENETIQUE
glucose fermentation
IMMOBILISATION
immobilized cells
INGENIERIA GENETICA
INMOVILIZACION
LACTOSA
LACTOSE
lactose fermentation
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
PRODUCCION
PRODUCTION
reverse osmosis membrane
Saccharomyces
SACCHAROMYCES CEREVISIAE
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Summary:A novel concept of membrane bioreactor in which living cells are sandwiched between ultrafiltration (UF) and reverse osmosis (RO) membranes was applied for lactose fermentation to ethanol by genetically engineered yeast cells. The productivity of the Lactophile 13B strains was higher than that of the Lactophile 13D strains. In both cases performance data similar to those for glucose fermentation to ethanol by Saccharomyces cerevisiae were obtained. However, the operational stability of recombinant yeast cells was improved in the new bioreactor in comparison to the stability of these cells in a shake flask
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260370614