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Origin of the chlorophyll b formyl oxygen in Chlorella vulgaris
Chlorophyll (Chl) b is an accessory light-harvesting pigment of plants and chlorophyte algae. Chl b differs from Chl a in that the 3-methyl group on ring B of Chl a is replaced by a 3-formyl group on Chl b. The present study determined the biosynthetic origin of the Chl b formyl oxygen in in vivo la...
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| Published in: | Biochemistry (Easton) 1992-12, Vol.31 (47), p.11677-11683 |
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| Main Authors: | , |
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| Language: | English |
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| container_end_page | 11683 |
| container_issue | 47 |
| container_start_page | 11677 |
| container_title | Biochemistry (Easton) |
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| creator | Schneegurt, Mark A Beale, Samuel I |
| description | Chlorophyll (Chl) b is an accessory light-harvesting pigment of plants and chlorophyte algae. Chl b differs from Chl a in that the 3-methyl group on ring B of Chl a is replaced by a 3-formyl group on Chl b. The present study determined the biosynthetic origin of the Chl b formyl oxygen in in vivo labeling experiments. A mutant strain of the unicellular chlorophyte Chlorella vulgaris, which can not synthesize Chls when cultured in the dark but rapidly greens when transferred to the light, was grown in the dark for several generations to deplete Chls, and then the cells were transferred to the light and allowed to form Chls in a controlled atmosphere containing (18)O2. Chl a and Chl b were purified from the cells and analyzed by high-resolution mass spectroscopy. Analysis of the mass spectra indicated that over 76% of the Chl a molecules had incorporated an atom of (18)O. For Chl b, 58% of the molecules had incorporated an atom of (18)O at one position and 34% of the molecules had incorporated an atom of (18)O at a second position. These results demonstrate that the isocyclic ring keto oxygen of both Chl a and Chl b, as well as the formyl oxygen of Chl b, is derived from O2 |
| doi_str_mv | 10.1021/bi00162a002 |
| format | article |
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Chl b differs from Chl a in that the 3-methyl group on ring B of Chl a is replaced by a 3-formyl group on Chl b. The present study determined the biosynthetic origin of the Chl b formyl oxygen in in vivo labeling experiments. A mutant strain of the unicellular chlorophyte Chlorella vulgaris, which can not synthesize Chls when cultured in the dark but rapidly greens when transferred to the light, was grown in the dark for several generations to deplete Chls, and then the cells were transferred to the light and allowed to form Chls in a controlled atmosphere containing (18)O2. Chl a and Chl b were purified from the cells and analyzed by high-resolution mass spectroscopy. Analysis of the mass spectra indicated that over 76% of the Chl a molecules had incorporated an atom of (18)O. For Chl b, 58% of the molecules had incorporated an atom of (18)O at one position and 34% of the molecules had incorporated an atom of (18)O at a second position. These results demonstrate that the isocyclic ring keto oxygen of both Chl a and Chl b, as well as the formyl oxygen of Chl b, is derived from O2</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00162a002</identifier><identifier>PMID: 1445904</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chlorella - chemistry ; CHLORELLA VULGARIS ; Chlorophyll - chemistry ; Chlorophyll - isolation & purification ; CHLOROPHYLLE ; CLOROFILAS ; ESPECTROMETRIA ; Fundamental and applied biological sciences. Psychology ; Heterocyclic compounds, pigments ; LUMIERE ; LUZ ; Mass Spectrometry ; Molecular Structure ; Molecular Weight ; OBSCURIDAD ; OBSCURITE ; Other biological molecules ; OXIGENO ; Oxygen - chemistry ; Oxygen Isotopes ; OXYGENE ; PROTEINAS ; PROTEINE ; SPECTROMETRIE ; Spectrophotometry ; Tetrapyrrolic pigments</subject><ispartof>Biochemistry (Easton), 1992-12, Vol.31 (47), p.11677-11683</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a431t-2e472e7704f9eefca11e139b91fe37ce46afe21ad3bc7a691be83572caf637f93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00162a002$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00162a002$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,27064,27924,27925,56766,56816</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4413659$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1445904$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schneegurt, Mark A</creatorcontrib><creatorcontrib>Beale, Samuel I</creatorcontrib><title>Origin of the chlorophyll b formyl oxygen in Chlorella vulgaris</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>Chlorophyll (Chl) b is an accessory light-harvesting pigment of plants and chlorophyte algae. Chl b differs from Chl a in that the 3-methyl group on ring B of Chl a is replaced by a 3-formyl group on Chl b. The present study determined the biosynthetic origin of the Chl b formyl oxygen in in vivo labeling experiments. A mutant strain of the unicellular chlorophyte Chlorella vulgaris, which can not synthesize Chls when cultured in the dark but rapidly greens when transferred to the light, was grown in the dark for several generations to deplete Chls, and then the cells were transferred to the light and allowed to form Chls in a controlled atmosphere containing (18)O2. Chl a and Chl b were purified from the cells and analyzed by high-resolution mass spectroscopy. Analysis of the mass spectra indicated that over 76% of the Chl a molecules had incorporated an atom of (18)O. For Chl b, 58% of the molecules had incorporated an atom of (18)O at one position and 34% of the molecules had incorporated an atom of (18)O at a second position. These results demonstrate that the isocyclic ring keto oxygen of both Chl a and Chl b, as well as the formyl oxygen of Chl b, is derived from O2</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chlorella - chemistry</subject><subject>CHLORELLA VULGARIS</subject><subject>Chlorophyll - chemistry</subject><subject>Chlorophyll - isolation & purification</subject><subject>CHLOROPHYLLE</subject><subject>CLOROFILAS</subject><subject>ESPECTROMETRIA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heterocyclic compounds, pigments</subject><subject>LUMIERE</subject><subject>LUZ</subject><subject>Mass Spectrometry</subject><subject>Molecular Structure</subject><subject>Molecular Weight</subject><subject>OBSCURIDAD</subject><subject>OBSCURITE</subject><subject>Other biological molecules</subject><subject>OXIGENO</subject><subject>Oxygen - chemistry</subject><subject>Oxygen Isotopes</subject><subject>OXYGENE</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>SPECTROMETRIE</subject><subject>Spectrophotometry</subject><subject>Tetrapyrrolic pigments</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqFkU1rFEEQhhtR4ho9eROEOYgeZLS_O30SWUxUAglkg8emplO9O7F3etO9I9l_by-zRA-Cp6J4Hoqqtwh5yegHRjn72PWUMs2BUv6IzJjitJXWqsdkRinVLbeaPiXPSrmtraRGHpEjJqWyVM7Ip4vcL_uhSaHZrrDxq5hy2qx2MTZdE1Je72KT7ndLHJpqzfcYY4Tm1xiXkPvynDwJEAu-ONRjcn36ZTH_2p5fnH2bfz5vQQq2bTlKw9EYKoNFDB4YQyZsZ1lAYTxKDQE5gxvReQPasg5PhDLcQ9DCBCuOydtp7ianuxHL1q374verDJjG4owQWkuj_isyrZimWlbx_ST6nErJGNwm92vIO8eo2-fq_sq12q8PY8dujTd_3CnIyt8cOBQPMWQYfF8eNCmZ0Gp_Rjtpfdni_QOG_NNpI4xyi8sr9_2HXZjTkzN3Wf1Xkx8gOVjWwN31lZVcM0UrfDdB8MXdpjEP9QP_3P43ubeiZg</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>Schneegurt, Mark A</creator><creator>Beale, Samuel I</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19921201</creationdate><title>Origin of the chlorophyll b formyl oxygen in Chlorella vulgaris</title><author>Schneegurt, Mark A ; Beale, Samuel I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a431t-2e472e7704f9eefca11e139b91fe37ce46afe21ad3bc7a691be83572caf637f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chlorella - chemistry</topic><topic>CHLORELLA VULGARIS</topic><topic>Chlorophyll - chemistry</topic><topic>Chlorophyll - isolation & purification</topic><topic>CHLOROPHYLLE</topic><topic>CLOROFILAS</topic><topic>ESPECTROMETRIA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heterocyclic compounds, pigments</topic><topic>LUMIERE</topic><topic>LUZ</topic><topic>Mass Spectrometry</topic><topic>Molecular Structure</topic><topic>Molecular Weight</topic><topic>OBSCURIDAD</topic><topic>OBSCURITE</topic><topic>Other biological molecules</topic><topic>OXIGENO</topic><topic>Oxygen - chemistry</topic><topic>Oxygen Isotopes</topic><topic>OXYGENE</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>SPECTROMETRIE</topic><topic>Spectrophotometry</topic><topic>Tetrapyrrolic pigments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schneegurt, Mark A</creatorcontrib><creatorcontrib>Beale, Samuel I</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schneegurt, Mark A</au><au>Beale, Samuel I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Origin of the chlorophyll b formyl oxygen in Chlorella vulgaris</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>31</volume><issue>47</issue><spage>11677</spage><epage>11683</epage><pages>11677-11683</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Chlorophyll (Chl) b is an accessory light-harvesting pigment of plants and chlorophyte algae. Chl b differs from Chl a in that the 3-methyl group on ring B of Chl a is replaced by a 3-formyl group on Chl b. The present study determined the biosynthetic origin of the Chl b formyl oxygen in in vivo labeling experiments. A mutant strain of the unicellular chlorophyte Chlorella vulgaris, which can not synthesize Chls when cultured in the dark but rapidly greens when transferred to the light, was grown in the dark for several generations to deplete Chls, and then the cells were transferred to the light and allowed to form Chls in a controlled atmosphere containing (18)O2. Chl a and Chl b were purified from the cells and analyzed by high-resolution mass spectroscopy. Analysis of the mass spectra indicated that over 76% of the Chl a molecules had incorporated an atom of (18)O. For Chl b, 58% of the molecules had incorporated an atom of (18)O at one position and 34% of the molecules had incorporated an atom of (18)O at a second position. These results demonstrate that the isocyclic ring keto oxygen of both Chl a and Chl b, as well as the formyl oxygen of Chl b, is derived from O2</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>1445904</pmid><doi>10.1021/bi00162a002</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemistry (Easton), 1992-12, Vol.31 (47), p.11677-11683 |
| issn | 0006-2960 1520-4995 |
| language | eng |
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| source | ACS CRKN Legacy Archives |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Chlorella - chemistry CHLORELLA VULGARIS Chlorophyll - chemistry Chlorophyll - isolation & purification CHLOROPHYLLE CLOROFILAS ESPECTROMETRIA Fundamental and applied biological sciences. Psychology Heterocyclic compounds, pigments LUMIERE LUZ Mass Spectrometry Molecular Structure Molecular Weight OBSCURIDAD OBSCURITE Other biological molecules OXIGENO Oxygen - chemistry Oxygen Isotopes OXYGENE PROTEINAS PROTEINE SPECTROMETRIE Spectrophotometry Tetrapyrrolic pigments |
| title | Origin of the chlorophyll b formyl oxygen in Chlorella vulgaris |
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