Model development for horseradish peroxidase catalyzed removal of aqueous phenol

Once activated by hydrogen peroxide, horseradish peroxidase (HRP) catalyzes the oxidation of aqueous aromatic compounds to produce high molecular weight polymers of low solubility. A pseudo steady‐state kinetic model of the HRP‐hydrogen peroxide‐aromatic compound system was modified to incorporate e...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1997-05, Vol.54 (3), p.251-261
Main Authors: Buchanan, Ian D., Nicell, James A.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
enzyme inactivation
Fundamental and applied biological sciences. Psychology
horseradish peroxidase
kinetic model
Methods. Procedures. Technologies
Miscellaneous
parameter estimation
phenol removal
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Summary:Once activated by hydrogen peroxide, horseradish peroxidase (HRP) catalyzes the oxidation of aqueous aromatic compounds to produce high molecular weight polymers of low solubility. A pseudo steady‐state kinetic model of the HRP‐hydrogen peroxide‐aromatic compound system was modified to incorporate enzyme inactivation mechanisms in order to improve its predictive ability. The kinetic constants of the model were calibrated using a series of experimental data sets. The model's ability to predict the time‐dependent removal of phenol within the range of 0.5–6 mM from a batch reactor was validated. The model accounts for permanent losses of enzyme activity through inactivation by free radicals as well as interaction with end‐product polymers as they form. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 251–261, 1997
ISSN:0006-3592
1097-0290
DOI:10.1002/(SICI)1097-0290(19970505)54:3<251::AID-BIT6>3.0.CO;2-E