Analysis of Changes in Cell Volume of a Mouse Early Embryo Exposed to Osmotic Shock

The impact of the osmotic component of the incubation medium for the volume of mouse early embryonic cell was studied by laser scanning microscopy. Common Dulbecco's medium caused a prolonged hyperosmotic effect. Adaptive phase of regulatory compensation for the osmotic shock was observed under...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2009-07, Vol.148 (1), p.152-154
Main Authors: Pogorelova, M. A, Goldshtein, D. V, Pogorelov, A. G, Golichenkov, V. A
Format: Article
Language:English
Subjects:
3D-reconstruction
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Size
Cell Technologies in Biology and Medicine
cell volume regulation
Embryo, Mammalian - cytology
Internal Medicine
Laboratory Medicine
laser scanning microscopy
Mice
Osmotic Pressure
osmotic stress
Pathology
two-cell mouse embryo
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Summary:The impact of the osmotic component of the incubation medium for the volume of mouse early embryonic cell was studied by laser scanning microscopy. Common Dulbecco's medium caused a prolonged hyperosmotic effect. Adaptive phase of regulatory compensation for the osmotic shock was observed under hypotonic conditions. From these data, water permeability of the blastomer membrane is evaluated as 0.4 μ/(min×atm).
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-009-0653-5