Microsatellite Marker Development and Mendelian Analysis in the Matschie's Tree Kangaroo (Dendrolagus matschiei)

Matschie's tree kangaroo (Dendrolagus matschiei) is an endangered arboreal macropodid endemic to the Huon Peninsula, Papua New Guinea (PNG). We developed 5 microsatellite markers for D. matschiei, which are the first markers developed for DENDROLAGUS: We screened 17 additional markers that were...

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Bibliographic Details
Published in:The Journal of heredity 2010-01, Vol.101 (1), p.113-118
Main Authors: McGreevy, Thomas J. Jr, Dabek, Lisa, Husband, Thomas P
Format: Article
Language:English
Subjects:
Animals
association of zoos and aquariums
Base Sequence
conservation genetics
Dendrolagus
Deoxyribonucleic acid
DNA
DNA Primers
Endangered & extinct species
endangered species
fecal DNA
Genetic diversity
Genetic Markers
Macropodidae - genetics
Marsupials
Microsatellite Repeats - genetics
noninvasive genotyping
Papua New Guinea
Wildlife conservation
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Summary:Matschie's tree kangaroo (Dendrolagus matschiei) is an endangered arboreal macropodid endemic to the Huon Peninsula, Papua New Guinea (PNG). We developed 5 microsatellite markers for D. matschiei, which are the first markers developed for DENDROLAGUS: We screened 17 additional markers that were developed for other marsupial taxa and identified 3 that were polymorphic in D. matschiei. We estimated allelic and genetic diversity with the set of 8 markers by analyzing 22 D. matschiei from Wasaunon on the Huon Peninsula, PNG. The number of alleles ranged from 2 to 9 and expected heterozygosity ranged from 0.440 to 0.794. We tested for null alleles and Mendelian inheritance by analyzing 19 pairs of D. matschiei parents and offspring from Association of Zoos and Aquariums institutions. Null alleles were not detected and Mendelian inheritance was followed for all 8 markers. We also evaluated the reliability of using the markers to amplify DNA extracted from D. matschiei fecal samples and the ability of the markers to amplify DNA samples from Goodfellow's tree kangaroo (Dendrolagus goodfellowi ssp.), Doria's tree kangaroo (Dendrolagus dorianus ssp.), and Grizzled tree kangaroo (Dendrolagus inustus ssp.). Microsatellite markers can be used to inform management decisions to conserve D. matschiei in captivity and the wild.
ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/esp080