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How to improve the success rate of mouse cloning technology
It has now been 13 years since the first cloned mammal Dolly the sheep was generated from somatic cells using nuclear transfer (SCNT). Since then, this technique has been considered an important tool not only for animal reproduction but also for regenerative medicine. However, the success rate is st...
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| Published in: | Journal of Reproduction and Development 2010, Vol.56(1), pp.20-30 |
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| container_title | Journal of Reproduction and Development |
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| creator | van Thuan, N., Konkuk Univ., Seoul (Korea R.) Kishigami, S Wakayama, T |
| description | It has now been 13 years since the first cloned mammal Dolly the sheep was generated from somatic cells using nuclear transfer (SCNT). Since then, this technique has been considered an important tool not only for animal reproduction but also for regenerative medicine. However, the success rate is still very low and the mechanisms involved in genomic reprogramming are not yet clear. Moreover, the NT technique requires donated fresh oocyte, which raises ethical problems for production of human cloned embryo. For this reason, the use of induced pluripotent stem cells for genomic reprogramming and for regenerative medicine is currently a hot topic in this field. However, we believe that the NT approach remains the only valid way for the study of reproduction and basic biology. For example, only the NT approach can reveal dynamic and global modifications in the epigenome without using genetic modification, and it can generate offspring from a single cell or even a frozen dead body. Thanks to much hard work by many groups, cloning success rates are increasing slightly year by year, and NT cloning is now becoming a more applicable method. This review describes how to improve the efficiency of cloning, the establishment of clone-derived embryonic stem cells and further applications. |
| doi_str_mv | 10.1262/jrd.09-221A |
| format | article |
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Since then, this technique has been considered an important tool not only for animal reproduction but also for regenerative medicine. However, the success rate is still very low and the mechanisms involved in genomic reprogramming are not yet clear. Moreover, the NT technique requires donated fresh oocyte, which raises ethical problems for production of human cloned embryo. For this reason, the use of induced pluripotent stem cells for genomic reprogramming and for regenerative medicine is currently a hot topic in this field. However, we believe that the NT approach remains the only valid way for the study of reproduction and basic biology. For example, only the NT approach can reveal dynamic and global modifications in the epigenome without using genetic modification, and it can generate offspring from a single cell or even a frozen dead body. Thanks to much hard work by many groups, cloning success rates are increasing slightly year by year, and NT cloning is now becoming a more applicable method. 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Reprod. Dev.</addtitle><description>It has now been 13 years since the first cloned mammal Dolly the sheep was generated from somatic cells using nuclear transfer (SCNT). Since then, this technique has been considered an important tool not only for animal reproduction but also for regenerative medicine. However, the success rate is still very low and the mechanisms involved in genomic reprogramming are not yet clear. Moreover, the NT technique requires donated fresh oocyte, which raises ethical problems for production of human cloned embryo. For this reason, the use of induced pluripotent stem cells for genomic reprogramming and for regenerative medicine is currently a hot topic in this field. However, we believe that the NT approach remains the only valid way for the study of reproduction and basic biology. For example, only the NT approach can reveal dynamic and global modifications in the epigenome without using genetic modification, and it can generate offspring from a single cell or even a frozen dead body. Thanks to much hard work by many groups, cloning success rates are increasing slightly year by year, and NT cloning is now becoming a more applicable method. This review describes how to improve the efficiency of cloning, the establishment of clone-derived embryonic stem cells and further applications.</description><subject>ANIMAL</subject><subject>ANIMALES</subject><subject>ANIMALS</subject><subject>Cellular Reprogramming - physiology</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>Clone</subject><subject>Clone Cells - physiology</subject><subject>CLONING</subject><subject>Cloning, Organism - methods</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Epigenesis, Genetic</subject><subject>Extinction, Biological</subject><subject>Female</subject><subject>GENETIC DISORDERS</subject><subject>GENETIC ENGINEERING</subject><subject>GENIE GENETIQUE</subject><subject>http://www.fao.org/aos/agrovoc#c_15952</subject><subject>http://www.fao.org/aos/agrovoc#c_15974</subject><subject>http://www.fao.org/aos/agrovoc#c_24120</subject><subject>http://www.fao.org/aos/agrovoc#c_3217</subject><subject>http://www.fao.org/aos/agrovoc#c_444</subject><subject>http://www.fao.org/aos/agrovoc#c_4795</subject><subject>INGENIERIA GENETICA</subject><subject>MALFORMACION</subject><subject>MALFORMATION</subject><subject>MALFORMATIONS</subject><subject>MICE</subject><subject>ntES cell</subject><subject>Nuclear Transfer</subject><subject>Nuclear Transfer Techniques</subject><subject>Oocytes - physiology</subject><subject>Pluripotent Stem Cells - physiology</subject><subject>RATON</subject><subject>Reprogramming</subject><subject>SOURIS</subject><subject>TRASTORNOS GENETICOS</subject><subject>TROUBLE GENETIQUE</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNo9kDtPwzAQgC0EgvKYmEHeGFDg_IjtiKlCPIUEA8yW61zaVEkMdgrqvyelpcvdcJ8-nT5CThlcMa749TyWV1BknLPxDhkxIU0mJcAuGUHBVGYMMwfkMKU5gOC5kvvkgAMHIQUfkZvH8EP7QOv2M4ZvpP0MaVp4jynR6HqkoaJtWCSkvgld3U1pj37WhSZMl8dkr3JNwpPNPiIf93fvt4_Zy-vD0-34JfPSmD6TTJqcKzMReSW0FlWelxyKIhe-dKrUUqCDCiZKc-9MyY2eVJo5h6AK0JqJI3Kx9g4vfi0w9batk8emcR0Or1kthNLCKDGQl2vSx5BSxMp-xrp1cWkZ2FUsO8SyUNhVrIE-33gXkxbLLftfZwDGa2CeejfFLeBiX_sG_2S5smw1NtLtzc9ctNgNjrO1o3LBummsk31-48AAgPNci18TX4Om</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>van Thuan, N., Konkuk Univ., Seoul (Korea R.)</creator><creator>Kishigami, S</creator><creator>Wakayama, T</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100201</creationdate><title>How to improve the success rate of mouse cloning technology</title><author>van Thuan, N., Konkuk Univ., Seoul (Korea R.) ; 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Reprod. Dev.</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>56</volume><issue>1</issue><spage>20</spage><epage>30</epage><pages>20-30</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>It has now been 13 years since the first cloned mammal Dolly the sheep was generated from somatic cells using nuclear transfer (SCNT). Since then, this technique has been considered an important tool not only for animal reproduction but also for regenerative medicine. However, the success rate is still very low and the mechanisms involved in genomic reprogramming are not yet clear. Moreover, the NT technique requires donated fresh oocyte, which raises ethical problems for production of human cloned embryo. For this reason, the use of induced pluripotent stem cells for genomic reprogramming and for regenerative medicine is currently a hot topic in this field. However, we believe that the NT approach remains the only valid way for the study of reproduction and basic biology. 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| ispartof | Journal of Reproduction and Development, 2010, Vol.56(1), pp.20-30 |
| issn | 0916-8818 1348-4400 |
| language | eng |
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| source | Directory of Open Access Journals |
| subjects | ANIMAL ANIMALES ANIMALS Cellular Reprogramming - physiology CLONACION CLONAGE Clone Clone Cells - physiology CLONING Cloning, Organism - methods Embryonic Stem Cells - physiology Epigenesis, Genetic Extinction, Biological Female GENETIC DISORDERS GENETIC ENGINEERING GENIE GENETIQUE http://www.fao.org/aos/agrovoc#c_15952 http://www.fao.org/aos/agrovoc#c_15974 http://www.fao.org/aos/agrovoc#c_24120 http://www.fao.org/aos/agrovoc#c_3217 http://www.fao.org/aos/agrovoc#c_444 http://www.fao.org/aos/agrovoc#c_4795 INGENIERIA GENETICA MALFORMACION MALFORMATION MALFORMATIONS MICE ntES cell Nuclear Transfer Nuclear Transfer Techniques Oocytes - physiology Pluripotent Stem Cells - physiology RATON Reprogramming SOURIS TRASTORNOS GENETICOS TROUBLE GENETIQUE |
| title | How to improve the success rate of mouse cloning technology |
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