Test compounds for detecting the silanol effect on the elution of ionized amines in reversed-phase LC

The effectiveness of several basic compounds for testing silica-based stationary phases was reviewed by applying them to recent columns for reversed-phase HPLC. Most octadecylsilylated (C18) stationary phases, prepared as a base-deactivated material from high-purity silica gel with endcapping, provi...

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Bibliographic Details
Published in:Journal of separation science 2010-02, Vol.33 (3), p.348-358
Main Authors: Okusa, Kensuke, Suita, Yuki, Otsuka, Yukio, Tahara, Mineo, Ikegami, Tohru, Tanaka, Nobuo, Ohira, Masayoshi, Takahashi, Masakazu
Format: Article
Language:English
Subjects:
Acceptability
Amines
Amines - chemistry
Amines - isolation & purification
Analysis
Analytical chemistry
Basic compound
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Chromatography, Reverse-Phase - instrumentation
Chromatography, Reverse-Phase - methods
Elution
Exact sciences and technology
General pharmacology
Ions - chemistry
Ions - isolation & purification
Medical sciences
Other chromatographic methods
Pharmacology. Drug treatments
Phases
Reversed-phase
Separation
Silanes - chemistry
Silanol effect
Silica gel
Silicon Dioxide - chemistry
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Summary:The effectiveness of several basic compounds for testing silica-based stationary phases was reviewed by applying them to recent columns for reversed-phase HPLC. Most octadecylsilylated (C18) stationary phases, prepared as a base-deactivated material from high-purity silica gel with endcapping, provided excellent peak shape and column efficiency for the bases including benzylamine and amitriptyline that once caused problems and were subsequently employed for testing silanol activities. However, a cyclic tertiary amine, dextrometorphan, was eluted as an acceptable peak from only a few columns at neutral pH. Such a more sensitive probe is expected to contribute to further improvement of the stationary phase for reversed-phase HPLC.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200900760