Constriction and Dnm1p recruitment are distinct processes in mitochondrial fission

Mitochondria undergo cycles of fusion and fission crucial for organelle homeostasis. Fission is regulated partially by recruitment of the large GTPase Dnm1p to the outer mitochondrial membrane. Using three-dimensional time-lapse fluorescence imaging of Saccharomyces cerevisiae cells, we found that D...

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Bibliographic Details
Published in:Molecular biology of the cell 2003-05, Vol.14 (5), p.1953-1963
Main Authors: Legesse-Miller, Aster, Massol, Ramiro H, Kirchhausen, Tom
Format: Article
Language:English
Subjects:
Genes, Reporter
GTP Phosphohydrolases - physiology
Mitochondria - physiology
Mitochondrial Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins - physiology
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Summary:Mitochondria undergo cycles of fusion and fission crucial for organelle homeostasis. Fission is regulated partially by recruitment of the large GTPase Dnm1p to the outer mitochondrial membrane. Using three-dimensional time-lapse fluorescence imaging of Saccharomyces cerevisiae cells, we found that Dnm1p-EGFP appears and disappears at "hot spots" along mitochondrial tubes. It forms patches that convert rapidly into different shapes regardless of whether mitochondrial fission ensues or not. Moreover, the thickness of the mitochondrial matrix displays frequent temporal fluctuations apparently unrelated to fission or to recruitment of Dnm1p-EGFP. These results suggest that mitochondrial fission requires coordination of at least two distinct processes.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e02-10-0657