PELDOR Spectroscopy Reveals Preorganization of the Neomycin-Responsive Riboswitch Tertiary Structure

Pulsed electron double resonance (PELDOR) spectroscopy reveals a prearranged tertiary structure of the 27 nucleotides long engineered neomycin-responsive riboswitch. Measured distances between spin labels at positions U4−U14, U4−U15, U14−U26, and U15−U26 were unchanged upon neomycin binding which im...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2010-02, Vol.132 (5), p.1454-1455
Main Authors: Krstić, Ivan, Frolow, Olga, Sezer, Deniz, Endeward, Burkhard, Weigand, Julia E, Suess, Beatrix, Engels, Joachim W, Prisner, Thomas F
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - metabolism
Electron Spin Resonance Spectroscopy - methods
Neomycin - metabolism
Nucleic Acid Conformation
Protein Binding
RNA - analysis
RNA - metabolism
Spin Labels
Uridine - analysis
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Summary:Pulsed electron double resonance (PELDOR) spectroscopy reveals a prearranged tertiary structure of the 27 nucleotides long engineered neomycin-responsive riboswitch. Measured distances between spin labels at positions U4−U14, U4−U15, U14−U26, and U15−U26 were unchanged upon neomycin binding which implies that the global stem-loop architecture is preserved in the absence and presence of the ligand. On the basis of our results, we infer that low-temperature PELDOR data unambiguously demonstrate the existence of an enthalpically favorable set of RNA conformations ready to bind the ligand without major global rearrangement.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja9077914