A Simple, Sensitive Determination of Ganciclovir in Infant Plasma by High-Performance Liquid Chromatography with Fluorescence Detection

This study developed a simple and sensitive method using reversed-phase high-performance liquid chromatography (HPLC) for ganciclovir (GCV) plasma concentrations in cytomegalovirus infectious infants with hearing loss. The method involves a simple protein precipitation procedure that uses no solid-p...

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Bibliographic Details
Published in:Journal of chromatographic science 2010-03, Vol.48 (3), p.208-211
Main Authors: Yoshida, Terumitsu, Takahashi, Ryohei, Imai, Koichi, Uchida, Hiroshi, Arai, Yasutoshi, Oh-ishi, Tsutomu
Format: Article
Language:English
Subjects:
Analysis
Antiviral Agents - blood
Biological and medical sciences
Chromatography, High Pressure Liquid - economics
Chromatography, High Pressure Liquid - methods
Chromatography, Reverse-Phase - economics
Chromatography, Reverse-Phase - methods
Fluorescence
Ganciclovir - blood
General pharmacology
Humans
Infant
Limit of Detection
Linear Models
Medical sciences
Pharmacology. Drug treatments
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Summary:This study developed a simple and sensitive method using reversed-phase high-performance liquid chromatography (HPLC) for ganciclovir (GCV) plasma concentrations in cytomegalovirus infectious infants with hearing loss. The method involves a simple protein precipitation procedure that uses no solid-phase or liquid-liquid extraction. The HPLC separation was carried out on a Cadenza CD-C18 column (3 µm, 4.6 mm × 150 mm) with phosphate buffer (pH 2.5, 25 mM) containing 1% methanol-acetonitrile mixture (4:3, v/v) as a mobile phase at a 0.7 mL/min flow rate. GCV was detected using a fluorescence detection (λex/em: 265/380 nm). The quantification limit was 0.025 µg/mL for 100 µL of plasma sample at which good intra- and inter-assay coefficient of variation values (µ 4.96%) and recoveries (94.9–96.5%) were established.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/48.3.208