Determination of aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed by ultra high-performance liquid chromatography-tandem mass spectrometry

A rapid and simple method was developed to determine aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed and pet foods by UHPLC-MS/MS. Because the complexity of the evaluated matrices, the proposed method is based on sonication extraction using an ACN/water mixture (80:20 v/v) followed by a cl...

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Bibliographic Details
Published in:Journal of separation science 2010-03, Vol.33 (4-5), p.502-508
Main Authors: López Grío, Sergio José, Garrido Frenich, Antonia, Martínez Vidal, José Luis, Romero-González, Roberto
Format: Article
Language:English
Subjects:
Aflatoxin B1 - analysis
Aflatoxins
Aflatoxins - analysis
Animal feed
Animal Feed - analysis
Biological and medical sciences
Carcinogens
Chromatography, High Pressure Liquid - methods
Extraction
Feed and pet food industries
Food Contamination
Food industries
Fundamental and applied biological sciences. Psychology
Linearity
Liquids
Matrices
Microbiology
MS/MS
Mycology
Mycotoxins
Mycotoxins - analysis
Ochratoxins - analysis
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Poisons
Separation
Tandem Mass Spectrometry - methods
Time Factors
UHPLC
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Summary:A rapid and simple method was developed to determine aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed and pet foods by UHPLC-MS/MS. Because the complexity of the evaluated matrices, the proposed method is based on sonication extraction using an ACN/water mixture (80:20 v/v) followed by a clean-up step utilising C₁₈ as sorbent. Performance parameters of the method were evaluated, including linearity, trueness, precision and LOQ. Good linearity was found for all mycotoxins, with determination coefficients higher than 0.99 in the range considered, using matrix-matched calibration for quantification purposes. Recoveries ranged from 84 to 113%, with RSD lower than 20%, whereas LOQs were 5 μg/kg for the assayed mycotoxins. Finally, the method was successfully applied to the analysis of 19 real samples, detecting aflatoxin G2 in two samples at 13 and 17 μg/kg respectively, whereas the other mycotoxins were detected at trace levels (
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200900663