Production, characterization and use of monoclonal antibodies to grapevine virus A

Four stable hybridoma cell lines secreting monoclonal antibodies to grapevine closterovirus A (GVA) were obtained by fusing spleen cells of immunized BALB/c mice with mouse myeloma cell line Sp 2/0-Ag 14. In ELISA all MAbs reacted with virus in leaf extracts from Nicotiana benthamiana, glasshouse-,...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 1992-01, Vol.127 (1/4), p.185-194
Main Authors: Boscia, D, Aslouj, E, Elicio, V, Savino, V, Castellano, M.A, Martelli, G.P
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Antibody Specificity
Epitopes
Fruit
grapevine virus A
monoclonal antibodies
Nicotiana benthamiana
Plant Diseases - microbiology
plant viruses
Plant Viruses - immunology
Serotyping
surface antigens
Vitis vinifera
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Four stable hybridoma cell lines secreting monoclonal antibodies to grapevine closterovirus A (GVA) were obtained by fusing spleen cells of immunized BALB/c mice with mouse myeloma cell line Sp 2/0-Ag 14. In ELISA all MAbs reacted with virus in leaf extracts from Nicotiana benthamiana, glasshouse-, field-, or in vitro-grown grapevines, or with cortical shavings from mature grape canes. In IEM tests, only one of the MAbs (PA 3.F5) decorated virus particles on the entire surface. This MAb was likely induced by a surface antigenic determinant, whereas the other three MAbs (PA 3.D11, PA 3.C6, and PA3.B9) were originated by cryptotopes. Coupling polyclonal antibodies for coating protein A-sensitized plates, and monoclonal antibody conjugates for antigen detection, gave highly efficient and reproducible results for identification of GVA in field-grown grapevines.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01309583