Adhesion of perichondrial cells to a polylactic acid scaffold

The number of chondrogenic cells available locally is an important factor in the repair process for cartilage defects. Previous studies demonstrated that the number of transplanted rabbit perichondrial cells (PC) remaining in a cartilage defect in vivo, after being carried into the site in a polylac...

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Bibliographic Details
Published in:Journal of orthopaedic research 2003-07, Vol.21 (4), p.584-589
Main Authors: Giurea, Alexander, Klein, Travis J, Chen, Albert C, Goomer, Randal S, Coutts, Richard D, Akeson, Wayne H, Amiel, David, Sah, Robert L
Format: Article
Language:English
Subjects:
Animals
Biomechanical Phenomena
Biomechanics
Cartilage - cytology
Cartilage - physiology
Cartilage repair
Cell Adhesion
Cell Transplantation
Collagen - analysis
Extracellular matrix
Extracellular Matrix - chemistry
Lactic Acid
Male
Membranes, Artificial
Perfusion
Perichondrium
Polyesters
Polymers
Rabbits
Tissue engineering
Tissue Engineering - methods
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Summary:The number of chondrogenic cells available locally is an important factor in the repair process for cartilage defects. Previous studies demonstrated that the number of transplanted rabbit perichondrial cells (PC) remaining in a cartilage defect in vivo, after being carried into the site in a polylactic acid (PLA) scaffold, declined markedly within two days. This study examined the ability of in vitro culture of PC/PLA constructs to enhance subsequent biomechanical stability of the cells and the matrix content in an in vitro screening assay. PC/PLA constructs were analyzed after 1 h, 1 and 2 weeks of culture. The biomechanical adherence of PC to the PLA scaffold was tested by subjecting the PC/PLA constructs to a range of flow velocities (0.25–25 mm/s), spanning the range estimated to occur under conditions of construct insertion in vivo. The adhesion of PC to the PLA carrier was increased significantly by 1 and 2 weeks of incubation, with 25 mm/s flow causing a 57% detachment of cells after 1 h of seeding, but only 7% and 16% after 1 and 2 weeks of culture, respectively ( p
ISSN:0736-0266
1554-527X
DOI:10.1016/S0736-0266(02)00263-2