Diversity of hypoviruses and other double-stranded RNAs in Cryphonectria parasitica in North America

Double-stranded (as) RNAs in Cryphonectria parasitica were randomly sampled from nine subpopulations in North America using an antibody-based detection system for dsRNA. dsRNA was detected in 166 (28%) of a total of 595 C parasitica isolates sampled by immunoblotting. Incidence of dsRNA infection wi...

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Bibliographic Details
Published in:Phytopathology 1997-10, Vol.87 (10), p.1026-1033
Main Authors: Peever, T.L, Liu, Y.C, Milgroom, M.G
Format: Article
Language:English
Subjects:
AGENT PATHOGENE
ARN
Biological and medical sciences
BIOLOGICAL CONTROL
CASTANEA DENTATA
Control
CONTROL BIOLOGICO
CONTROL DE ENFERMEDADES
CONTROLE DE MALADIES
CRYPHONECTRIA PARASITICA
DISEASE CONTROL
ENFERMEDADES FUNGOSAS
Fundamental and applied biological sciences. Psychology
FUNGAL DISEASES
Fungal plant pathogens
GENETIC ANALYSIS
GENETIC VARIATION
GENETICA
GENETICS
GENETIQUE
GENOTIPOS
GENOTYPE
GENOTYPES
HIBRIDACION
HIPERPARASITISMO
HYBRIDATION
HYBRIDIZATION
HYPERPARASITISM
HYPERPARASITISME
KENTUCKY
LUTTE BIOLOGIQUE
MALADIE FONGIQUE
MARYLAND
MICHIGAN
NEW HAMPSHIRE
NEW JERSEY
NEW YORK
NUEVA YORK
ONTARIO
ORGANISMOS PATOGENOS
PATHOGENS
PATHOTYPE
PATHOTYPES
PATOTIPOS
Phytopathology. Animal pests. Plant and forest protection
PLANT VIRUSES
RNA
VARIACION GENETICA
VARIATION GENETIQUE
VIRGINIE OCCIDENTALE
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
WEST VIRGINIA
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Description
Summary:Double-stranded (as) RNAs in Cryphonectria parasitica were randomly sampled from nine subpopulations in North America using an antibody-based detection system for dsRNA. dsRNA was detected in 166 (28%) of a total of 595 C parasitica isolates sampled by immunoblotting. Incidence of dsRNA infection within subpopulations ranged from 0% in samples from New Hampshire and Ontario to 100% in County Line, MI. Most of the dsRNAs sampled were approximately 9 to 13 kb in size. dsRNAs from 72 isolates analyzed by probing Northern blots with 32P-labeled dsRNAs were in one of three hybridization groups. One hybridization group was widespread throughout eastern North America, being found in New York, New Jersey, Maryland, West Virginia, Kentucky, and Michigan. These dsRNAs hybridized to dsRNA from the previously described C. parasitica isolate SR2 from Maryland and are referred to as SR2-type dsRNAs. The second hybridization group was found almost exclusively in Michigan. The Michigan dsRNAs cross-hybridized to Cryphonectria hypovirus 3-GH2 (CHV3-GH2) and are referred to as CHV3-type dsRNAs. One dsRNA sampled from Kentucky hybridized to CHV3-type dsRNAs from Michigan. This dsRNA was probably derived from a fungal isolate that had been intentionally released for biological control at this same site 10 years previously and had become established in Kentucky. The third hybridization group was found only in New Jersey. These dsRNAs were much smaller than all other dsRNAs (3 and 5 kb) and were found in all 11 isolates that were probed; two of these isolates also had SR2-type dsRNA in mixed infections. None of the North American dsRNAs hybridized to CHV1 from Europe, even though CHV1 has been released in numerous locations in eastern North America for biological control of chestnut blight. Similarly, no dsRNAs hybridized to CHV2-NB58, a hypovirus found previously in New Jersey
ISSN:0031-949X
1943-7684
DOI:10.1094/phyto.1997.87.10.1026