class IV chitinase is up-regulated by fungal infection and abiotic stresses and associated with slow-canker-growth resistance to Cronartium ribicola in western white pine (Pinus monticola)

In the present study, in a candidate gene approach, a class IV chitinase gene (PmCh4A) of pathogenesis-related family three was cloned and characterized in western white pine (Pinus monticola). PmCh4A chitinase expression in the different organs of healthy seedlings was below levels detectable by we...

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Bibliographic Details
Published in:Phytopathology 2005-03, Vol.95 (3), p.284-291
Main Authors: Liu, J.J, Ekramoddoullah, A.K.M, Zamani, A
Format: Article
Language:English
Subjects:
abiotic stress
amino acid sequences
Biological and medical sciences
cankers (plants)
chitinase
complementary DNA
conifer needles
Cronartium ribicola
defense mechanisms
disease resistance
forest trees
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
Fungal plant pathogens
gene expression
genes
molecular sequence data
pathogenicity
Phytopathology. Animal pests. Plant and forest protection
Pine trees
Pinus monticola
plant pathogenic fungi
protein synthesis
stress tolerance
symptoms
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Summary:In the present study, in a candidate gene approach, a class IV chitinase gene (PmCh4A) of pathogenesis-related family three was cloned and characterized in western white pine (Pinus monticola). PmCh4A chitinase expression in the different organs of healthy seedlings was below levels detectable by western immunoblot analysis using an antibody raised against PmCh4A protein. However, a 27-kDa isozyme of PmCh4A accumulated in both susceptible and slow-canker-growth (SCG) resistant seedlings after infection by Cronartium ribicola. As with fungal infection, the application of a signal chemical (methyl jasmonate) and a protein phosphatase 1 and 2A inhibitor (okadaic acid) increased the PmCh4A protein accumulation. Furthermore, another 26-kDa isozyme was expressed specifically in SCG resistant seedlings, providing a potential tool for marker-assisted selection in forest breeding. Wounding treatment also induced expression of the protein. These data suggest that the class IV chitinase PmCh4A is involved in the defense response of western white pine to infection and abiotic stresses.
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-95-0284