Variability among turnip mosaic potyvirus isolates

Eight turnip mosaic potyvirus (TuMV) isolates from the Campania region of Italy were characterized. Experiments based on host range and symptomatology indicated that the isolates were biologically different. In addition, the isolates, with the exception of ITA1 and ITA3, were distinguished from each...

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Bibliographic Details
Published in:Phytopathology 1998-11, Vol.88 (11), p.1200-1204
Main Authors: Stavolone, L, Alioto, D, Ragozzino, A, Laliberte, J.F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Brassica
brassica campestris var. septices
Brassica rapa subsp. campestris
Brassica rapa subsp. rapa
Brassica ruvo
Erysimum cheiri
Fundamental and applied biological sciences. Psychology
geographical races
host range
Matthiola incana
Papaver rhoeas
pathogenicity
pathotypes
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Raphanus sativus
symptoms
Systematics. Structure, properties and multiplication. Genetics
Turnip mosaic virus
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Summary:Eight turnip mosaic potyvirus (TuMV) isolates from the Campania region of Italy were characterized. Experiments based on host range and symptomatology indicated that the isolates were biologically different. In addition, the isolates, with the exception of ITA1 and ITA3, were distinguished from each other by using a combination of monoclonal antibodies recognizing the coat protein. Single-strand conformation polymorphism (SSCP) analysis of the coat protein gene revealed that each isolate produced a specific SSCP profile, except for isolates ITA1 and ITA3. This study indicates that (i) even in a small geographical region, there is a great deal of variation in TuMV isolates; (ii) the use of a set of four differential hosts does not always specify the same pathotype in different environments; (iii) the TuMV isolates with the same pathotype on Brassica napus test lines can still differ in host range, symptoms, serology, and SSCP; and (iv) there was perfect correlation between the panel of antibodies and SSCP in differentiating among the isolates; ITA1 and ITA3 were indistinguishable by either assay.
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO.1998.88.11.1200