Resistance to Aspergillus flavus in corn kernels is associated with a 14-kDa protein

Corn genotypes resistant or susceptible to Aspergillus flavus were extracted for protein analysis using a pH 2.8 buffer. The profile of protein extracts revealed that a 14-kDa protein is present in relatively high concentrattion in kernels of seven resistant corn genotypes, but is absent or present...

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Bibliographic Details
Published in:Phytopathology 1998-04, Vol.88 (4), p.276-281
Main Authors: Chen, Z.Y. (Louisiana State University, Baton Rouge, New Orleans.), Brown, R.L, Lax, A.R, Guo, B.Z, Cleveland, T.E, Russin, J.S
Format: Article
Language:English
Subjects:
AFLATOXINAS
AFLATOXINE
AFLATOXINS
AGENT PATHOGENE
Agronomy. Soil science and plant productions
AMINO ACID SEQUENCES
ASPERGILLUS FLAVUS
Biological and medical sciences
CHEMICAL COMPOSITION
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DISEASE RESISTANCE
ENFERMEDADES FUNGOSAS
ENSAYO
Fundamental and applied biological sciences. Psychology
FUNGAL DISEASES
GENETIC VARIATION
Genetics and breeding of economic plants
GENOTIPOS
GENOTYPE
GENOTYPES
MALADIE FONGIQUE
MOLECULAR SEQUENCE DATA
ORGANISMOS PATOGENOS
PATHOGENICITY
PATHOGENS
PATOGENICIDAD
Pest resistance
Plant pathogens
PLANT PROTEINS
POUVOIR PATHOGENE
PROTEIN SYNTHESIS
PROTEINAS
PROTEINE
PROTEINS
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
SCREENING
SINTESIS DE PROTEINAS
SUSCEPTIBILITY
SYNTHESE PROTEIQUE
TESTAGE
TESTING
VARIACION GENETICA
VARIATION GENETIQUE
Varietal selection. Specialized plant breeding, plant breeding aims
ZEA MAYS
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Description
Summary:Corn genotypes resistant or susceptible to Aspergillus flavus were extracted for protein analysis using a pH 2.8 buffer. The profile of protein extracts revealed that a 14-kDa protein is present in relatively high concentrattion in kernels of seven resistant corn genotypes, but is absent or present only in low concentration in kernels of six susceptible ones. The N-terminal sequence of this 14-kDa protein showed 100% homology to a corn trypsin inhibitor. The 14-kDa protein purified from resistant varieties also demonstrated in vitro inhibition of both trypsin activity and the growth of A. flavus. This is the first demonstration of antifungal activity of a corn 14-kDa trypsin inhibitor protein. The expression of this protein among tested genotypes may be related to their difference in resistance to A. flavus infection and subsequent aflatoxin contamination
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO.1998.88.4.276