Survey methods for assessment of citrus tristeza virus incidence

The assessment of citrus tristeza virus incidence by sampling involves laboratory testing by enzyme-linked immunosorbent assay of leaf material collected in the field. Using field data and computer simulation, methods of field data collection were compared. One method was similar to that used by the...

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Bibliographic Details
Published in:Phytopathology 1998-07, Vol.88 (7), p.715-723
Main Authors: Hughes, G. (University of Edinburgh, Scotland, UK.), Gottwald, T.R
Format: Article
Language:English
Subjects:
Biological and medical sciences
CITRUS TRISTEZA CLOSTEROVIRUS
CLOSTEROVIRUS TRISTEZA DEL CITRUS
CLOSTEROVIRUS TRISTEZA DU CITRUS
CLUSTER ANALYSIS
DISEASE SURVEYS
DISTRIBUCION ESPACIAL
DISTRIBUTION SPATIALE
ECHANTILLONNAGE
ENCUESTAS SANITARIAS
ENQUETE PATHOLOGIQUE
EQUATIONS
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
MATEMATICAS
MATHEMATICAL MODELS
MATHEMATICS
MATHEMATIQUE
METHODE
METHODE STATISTIQUE
METHODOLOGY
METHODS
METODOS
METODOS ESTADISTICOS
MODELE MATHEMATIQUE
MODELOS MATEMATICOS
MUESTREO
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
SAMPLING
SPATIAL DISTRIBUTION
STATISTICAL METHODS
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Description
Summary:The assessment of citrus tristeza virus incidence by sampling involves laboratory testing by enzyme-linked immunosorbent assay of leaf material collected in the field. Using field data and computer simulation, methods of field data collection were compared. One method was similar to that used by the Central California Tristeza Eradication Agency, in which 4 to 6% of the trees in a planting block are sampled and material from each tree sampled is assayed separately. This method was compared with an alternative method in which about 25% of the trees in a block are sampled, and material from groups of four trees is bulked and assayed together. Our comparative study indicated that the latter method results in increased accuracy and precision of estimates of citrus tristeza virus incidence without increasing unduly the number of laboratory assays required
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO.1998.88.7.715