Osmotin and thaumatin from grape: a putative general defense mechanism against pathogenic fungi

Little information is available concerning the expression of pathogenesis-related (PR) proteins in grapevine (Vitis vinifera) and their effect properties on the major fungal pathogens of grape. A systematic study was performed on the effect of total or individual grape proteins on mycelial growth, s...

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Bibliographic Details
Published in:Phytopathology 2003-12, Vol.93 (12), p.1505-1512
Main Authors: Monteiro, S, Barakat, M, Picarra-Pereira, M.A, Teixeira, A.R, Ferreira, R.B
Format: Article
Language:English
Subjects:
antifungal properties
Biological and medical sciences
Botrytis cinerea
cell growth
defense mechanisms
disease resistance
fruits (plant anatomy)
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
germ cells
leaves
mycelium
Osmotin
pathogenesis-related proteins
pathogenicity
Phomopsis viticola
Phytopathology. Animal pests. Plant and forest protection
plant pathogenic fungi
PR protein
spore germination
symptoms
Thaumatin
Uncinula necator
Vitis vinifera
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Summary:Little information is available concerning the expression of pathogenesis-related (PR) proteins in grapevine (Vitis vinifera) and their effect properties on the major fungal pathogens of grape. A systematic study was performed on the effect of total or individual grape proteins on mycelial growth, spore germination, and germ tube growth of Uncinula necator, Phomopsis viticola, and Botrytis cinerea. Two proteins, identified as PR proteins by immunological methods and by N-terminal sequencing as osmotin and thaumatin-like protein, exhibited strong antifungal activities in vitro, blocking the growth of Phomopsis viticola and Botrytis cinerea mycelia. In addition, they inhibited spore germination and germ tube growth of U. necator, Phomopsis viticola, and Botrytis cinerea. The presence of both proteins displayed a synergistic effect. The expression of osmotin and thaumatin-like protein was induced in grapevine leaves and berries infected with U. necator and Phomopsis viticola. Thaumatin previously was thought to occur exclusively in berries. Immunoblot analyses revealed the accumulation of the two PR proteins in infected leaves and berries, supporting a role in vivo in increasing the resistance of grapevine to fungal attack.
ISSN:0031-949X
1943-7684
DOI:10.1094/phyto.2003.93.12.1505