First detection of genotype 3 hepatitis E virus RNA in river water in Cambodia

To identify and describe hepatitis E virus (HEV) strains in aquatic environments in Cambodia, we collected two river-water samples from upstream and downstream sites along the Siem Reap River and examined them for HEV using a virus concentration method followed by TaqMan reverse transcription PCR an...

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Bibliographic Details
Published in:Transactions of the Royal Society of Tropical Medicine and Hygiene 2009-09, Vol.103 (9), p.955-957
Main Authors: Kitajima, Masaaki, Matsubara, Koichi, Sour, Sethy, Haramoto, Eiji, Katayama, Hiroyuki, Ohgaki, Shinichiro
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cambodia
Environmental microbiology
Environmental Monitoring
Freshwater
General aspects
Genotype
Hepatitis E virus
Hepatitis E virus - classification
Hepatitis E virus - genetics
Hepatitis E virus - isolation & purification
Medical sciences
Phylogeny
Public health
Reverse Transcriptase Polymerase Chain Reaction - methods
Rivers
Rivers - virology
RNA, Viral - genetics
RT-PCR
Sequence Analysis, DNA
Water Microbiology
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Summary:To identify and describe hepatitis E virus (HEV) strains in aquatic environments in Cambodia, we collected two river-water samples from upstream and downstream sites along the Siem Reap River and examined them for HEV using a virus concentration method followed by TaqMan reverse transcription PCR and genetic analysis of the PCR product. Genotype 3 HEV strain was detected from the river-water sample collected from the river downstream, indicating that genotype 3 HEV strains are circulating in this region. To our knowledge, this is the first report demonstrating HEV contamination in aquatic environments in Cambodia.
ISSN:0035-9203
1878-3503
DOI:10.1016/j.trstmh.2009.04.004