Stability of diacylglycerol acyltransferase in dehydrated bovine muscle tissue

Meaningful estimates of diacylglycerol acyltransferase (EC 2.3.1.20) activity in different tissue samples require effective, unbiased methods of sample storage. Samples of the pars costalis diaphragmatis muscle (skirt muscle of the diaphragm) were obtained from 18- to 20-month-old cattle and assayed...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 2003-07, Vol.318 (2), p.254-259
Main Authors: Huff, Phillip W, Lozeman, Fred J, Chris Kazala, E, Prozniak, Cara, Wegner, Jochen, Deng, Jingti, Laroche, André, Mir, Priya S, Aalhus, Jennifer, Weselake, Randall J
Format: Article
Language:English
Subjects:
Acyltransferases - chemistry
Acyltransferases - metabolism
Animals
Bos taurus
Cattle
Desiccation
Diacylglycerol O-Acyltransferase
Diaphragm - enzymology
Enzyme Stability
Protein Conformation
Tissue storage
Triacylglycerol biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Meaningful estimates of diacylglycerol acyltransferase (EC 2.3.1.20) activity in different tissue samples require effective, unbiased methods of sample storage. Samples of the pars costalis diaphragmatis muscle (skirt muscle of the diaphragm) were obtained from 18- to 20-month-old cattle and assayed for microsomal protein content and diacylglycerol acyltransferase activity after having been stored under various conditions as dissected tissue or microsomes prepared from dissected tissue. There was relative enrichment of diacylglycerol acyltransferase specific activity ( p
ISSN:0003-2697
1096-0309
DOI:10.1016/S0003-2697(03)00216-1