Reconstitution of the leucine transport system of Lactococcus lactis into liposomes composed of membrane-spanning lipids from Sulfolobus acidocaldarius

The effect of bipolar tetraether lipids, extracted from the thermophilic archaebacterium Sulfolobus acidocaldarius, on the branched-chain amino acid transport system of the mesophilic bacterium Lactococcus lactis was investigated. Liposomes were prepared from mixtures of monolayer lipids and the bil...

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Published in:Biochemistry (Easton) 1992-12, Vol.31 (49), p.12493-12499
Main Authors: In't Veld, Gerda, Elferink, Marieke G. L, Driessen, Arnold J. M, Konings, Wil N
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Biological Transport
Calorimetry, Differential Scanning
Centrifugation, Density Gradient
Fluorescence Polarization
Fundamental and applied biological sciences. Psychology
Lactococcus lactis - metabolism
leucine
Leucine - metabolism
Lipid Bilayers - metabolism
lipids
liposomes
Liposomes - metabolism
Membrane Fluidity
Membrane Fusion
Membrane Lipids - metabolism
Microbiology
Permeability, membrane transport, intracellular transport
Sulfolobus acidocaldarius
Sulfolobus acidocaldarius - metabolism
transport
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Summary:The effect of bipolar tetraether lipids, extracted from the thermophilic archaebacterium Sulfolobus acidocaldarius, on the branched-chain amino acid transport system of the mesophilic bacterium Lactococcus lactis was investigated. Liposomes were prepared from mixtures of monolayer lipids and the bilayer lipid phosphatidylcholine (PC), analyzed on their miscibility, and fused with membrane vesicles from L. lactis. Freeze-fracture electron microscopy demonstrates that the bipolar lipids in the hybrid membranes adopted a monomolecular organization at high S. acidocaldarius lipid content. Leucine transport activity (i.e., delta mu H(+)-driven and counterflow uptake) increased with the content of S. acidocaldarius lipids and was optimal at a one-to-one (w/w) ratio of PC to S. acidocaldarius lipids. Membrane fluidity decreased with increasing S. acidocaldarius lipid content. These data suggest that transport proteins can be functionally reconstituted into membranes composed of membrane-spanning lipids provided that membrane viscosity is restricted.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00164a028