Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus

Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which causes significant economic losses to the pig industry worldwide. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. This study was aimed to develop a recombinant human adenovirus...

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Bibliographic Details
Published in:Research in veterinary science 2010-02, Vol.88 (1), p.77-82
Main Authors: Sun, Yuan, Liu, Da-Fei, Wang, Yu-Fei, Liang, Bing-Bing, Cheng, Dan, Li, Na, Qi, Qiao-Fen, Zhu, Qing-Hu, Qiu, Hua-Ji
Format: Article
Language:English
Subjects:
Adenoviridae
Adenoviridae - genetics
Adenoviridae - immunology
Adenovirus
Animals
antibodies
Antibodies, Viral - immunology
Blotting, Western
Classical Swine Fever - immunology
Classical Swine Fever - prevention & control
Classical Swine Fever - virology
Classical swine fever virus
Classical swine fever virus - immunology
E2 gene
Efficacy
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique, Indirect
Genetic Vectors - genetics
glycoproteins
hog cholera
Human adenovirus
immunization
Microbiology
Pig
protein synthesis
Proteins
Rabbit
rabbits
Rabbits - immunology
Recombinant adenovirus
Reverse Transcriptase Polymerase Chain Reaction
swine
Swine - immunology
Swine - virology
swine diseases
Vaccines
Vaccines, Synthetic - immunology
Vaccines, Synthetic - therapeutic use
Veterinary medicine
Viral Envelope Proteins - biosynthesis
Viral Envelope Proteins - immunology
Viruses
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Summary:Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which causes significant economic losses to the pig industry worldwide. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. This study was aimed to develop a recombinant human adenovirus type 5 expressing the CSFV E2 gene (rAdV-E2) and evaluate its efficacy in rabbits and pigs. The results showed that the rabbits and the pigs immunized with the rAdV-E2 developed high-level CSFV-specific neutralizing antibodies. The rAdV-E2-immunized rabbits were protected from fever induced by infection with C-strain, which is pathogenic to the rabbit, and the rAdV-E2-immunized pigs were protected from lethal challenge with highly virulent Shimen strain. This indicates that the recombinant adenovirus can be an attractive candidate vaccine for preventing CSF.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2009.06.005