Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy

Atomic force microscopy applications extend across a number of fields; however, limitations have reduced its effectiveness in live cell analysis. This report discusses the use of AFM to evaluate the three-dimensional (3-D) architecture of living chick dorsal root ganglia and sympathetic ganglia. The...

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Bibliographic Details
Published in:Journal of neuroscience methods 2005-03, Vol.142 (2), p.177-184
Main Authors: McNally, Helen A., Rajwa, Bartek, Sturgis, Jennie, Robinson, J. Paul
Format: Article
Language:English
Subjects:
Animals
Atomic force microscopy
Cells, Cultured
Chick Embryo
Confocal laser scanning microscopy
Ganglia, Spinal - cytology
Ganglia, Spinal - physiology
Ganglia, Spinal - ultrastructure
Image Processing, Computer-Assisted - methods
Imaging, Three-Dimensional - methods
Microscopy, Atomic Force - methods
Microscopy, Confocal - methods
Neurobiology
Neurogenesis
Neurons - cytology
Neurons - physiology
Neurons - ultrastructure
Neurotrauma
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Summary:Atomic force microscopy applications extend across a number of fields; however, limitations have reduced its effectiveness in live cell analysis. This report discusses the use of AFM to evaluate the three-dimensional (3-D) architecture of living chick dorsal root ganglia and sympathetic ganglia. These data sets were compared to similar images acquired with confocal laser scanning microscopy of identical cells. For this comparison we made use of visualization techniques which were applicable to both sets of data and identified several issues when coupling these technologies. These direct comparisons offer quantitative validation and confirmation of the character of novel images acquired by AFM. This paper is one in a series emphasizing various new applications of AFM in neurobiology.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2004.08.018