Osteochondral Integration of Multiply Incised Pure Cartilage Allograft: Repair Method of Focal Chondral Defects in a Porcine Model

• Published in: The American journal of sports medicine 2009-11, Vol.37 (1_suppl), p.50-57
• Main Authors: Bardos, Tamas, Farkas, Boglarka, Mezes, Beata, Vancsodi, Jozsef, Kvell, Krisztian, Czompoly, Tamas, Nemeth, Peter, Bellyei, Arpad, Illes, Tamas
• Format: Article
• Language: English
• Subjects: Animals; Arthroscopy; Cartilage; Cartilage - transplantation; Cartilage, Articular - injuries; Cartilage, Articular - surgery; Knee Injuries - surgery; Laboratory animals; Orthopedic Procedures - methods; Outcome Assessment (Health Care); Skin & tissue grafts; Surgical techniques; Swine; Transplantation, Homologous
• ISSN: 0363-5465; 1552-3365
• DOI: 10.1177/0363546509350045

Background A focal cartilage lesion has limited capacity to heal, and the repair modalities used at present are still unable to provide a universal solution. Pure cartilage graft implantation appears to be a simple option, but it has not been applied widely as cartilage will not reattach easily to the subchondral bone. Hypothesis We used a multiple-incision technique (processed chondrograft) to increase cartilage graft surface. We hypothesized that pure cartilage graft with augmented osteochondral fusion capacity may be used for cartilage repair and we compared this method with other repair techniques. Study Design Controlled laboratory study. Methods Full-thickness focal cartilage defects were created on the medial femoral condyle of 9-month-old pigs; defects were repaired using various methods including bone marrow stimulation, autologous chondrocyte implantation, and processed chon-drograft. After the repair, at weeks 6 and 24, macroscopic and histologic evaluation was carried out. Results Compared with other methods, processed chondrograft was found to besimilarly effective incartilage repair. Defects without repair and defects treated with bone marrow stimulation appeared slightly irregular with fibrocartilage filling. Autologous chondrocyte implantationproducedhyalinelikecartilage, althoughitscellularorganizationwasdistinguishablefromthesurroundingarticularcartilage. Processed chondrograft demonstrated good osteochondral integration, and the resulting tissue appeared to be hyaline cartilage. Conclusion The applied cartilage surface processing method allows acceptable osteochondral integration, and the repair tissue appears to have good macroscopic and histologic characteristics. Clinical Relevance If further studies confirm its efficacy, this technique could be considered for human application in the future.