Effect of Linseed on Intramuscular Fat Content and Adipogenesis Related Genes in Skeletal Muscle of Pigs

• Published in: Lipids 2009-11, Vol.44 (11), p.999-1010
• Main Authors: Luo, He-Feng, Wei, Hong-Kui, Zhou, Zheng, Jiang, Si-Wen, Peng, Jian
• Format: Article
• Language: English
• Subjects: Adipogenesis; Adipogenesis - genetics; Animals; Biomedical and Life Sciences; Diet; dietary fat; dietary supplements; Enzymatic activity; fatty acid composition; Fatty Acid-Binding Proteins - genetics; Fatty Acid-Binding Proteins - metabolism; Fatty acids; Fatty Acids - metabolism; Fatty Acids, Omega-3 - metabolism; finishing; Flax; gene expression regulation; Intramuscular fat; Life Sciences; Linseed; Linum usitatissimum; lipid content; Lipidology; lipogenesis; Lipoprotein Lipase - genetics; Lipoprotein Lipase - metabolism; longissimus dorsi; meat quality; Medical Biochemistry; Medicinal Chemistry; Microbial Genetics and Genomics; Muscle, Skeletal - metabolism; Neurochemistry; Nutrition; n‐3 PUFA; omega-3 fatty acids; Original Article; peroxisome proliferator-activated receptors; polyunsaturated fatty acids; PPAR delta - genetics; PPAR delta - metabolism; PPAR gamma - genetics; PPAR gamma - metabolism; PPARγ; PPARδ; Swine; swine feeding; transcription (genetics); Wnt10b
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-009-3346-y

The aim of the study was to investigate the effect of n-3 PUFA enrichment in longissimus muscle on intramuscular fat (IMF) content and expression of related genes in growing-finishing barrows. Two isoenergetic, isonitrogenous and isolipidic diets were formulated: one was basal diet and the other contained 10% linseed. Twenty-four Landrace × NewDamLine barrows weighing 35 ± 3.7 kg were randomly assigned to four treatment groups with six pigs per group. During the whole experimental period of 90 days, all groups were first fed the basal diet and then the linseed diet for 0, 30, 60, and 90 days before slaughter, respectively. Meat quality, fatty acid composition, and expression of genes involved in adipogenesis in longissimus muscle were measured and analyzed. The IMF content increased linearly (P < 0.05) as the linseed diet feeding time prolonged. Meanwhile, n-3 PUFA content and expression of peroxisome proliferator-activated receptor δ (PPARδ), PPARγ, adipocyte fatty acid-binding protein (aP2) and lipoprotein lipase (LPL) increased linearly (P < 0.01) as well, while the expression of wingless related MMTV integration site 10b (Wnt10b) linearly decreased (P < 0.01). Furthermore, significant (P < 0.01) quadratic or linear relation was observed between n-3 PUFA enrichment and expression of these genes, while significant (P < 0.01) quadratic or linear relation was observed between the expression of PPARγ, aP2 or Wnt10b and IMF content. These data show that enhancing n-3 PUFA enrichment in muscle leads to significant increase in IMF content. A possible explanation is due to alterations in the expression of genes involved in adipogenesis, however this will need to be confirmed by protein and enzyme activity studies.