Metalloproteinase Expression is Associated with Traumatic Wound Failure

Background Matrix metalloproteinases (MMPs) are crucial in the inflammatory and remodeling phases of wound healing. We previously reported the correlation between pro-inflammatory cytokines and timing of successful combat-wound closure. We now extend our studies to investigate the correlation betwee...

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Bibliographic Details
Published in:The Journal of surgical research 2010-04, Vol.159 (2), p.633-639
Main Authors: Utz, Edward R., B.S, Elster, Eric A., M.D, Tadaki, Douglas K., Ph.D, Gage, Frederick, Perdue, Philip W., M.D, Forsberg, Jonathan A., M.D, Stojadinovic, Alexander, M.D, Hawksworth, Jason S., M.D, Brown, Trevor S., Ph.D
Format: Article
Language:English
Subjects:
acute wound
Adolescent
Adult
Amputation - statistics & numerical data
Biological and medical sciences
Debridement
dehiscence
effluent
Female
General aspects
Humans
Luminex
Male
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 3 - genetics
Matrix Metalloproteinase 7 - genetics
Matrix Metalloproteinases - genetics
Medical sciences
Military Medicine - methods
MMP-2
MMP-3
MMP-7
multiplex
Prospective Studies
Surgery
VAC
Wound Healing - physiology
Wounds and Injuries - enzymology
Wounds and Injuries - genetics
Wounds and Injuries - surgery
Wounds, Penetrating - enzymology
Young Adult
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Summary:Background Matrix metalloproteinases (MMPs) are crucial in the inflammatory and remodeling phases of wound healing. We previously reported the correlation between pro-inflammatory cytokines and timing of successful combat-wound closure. We now extend our studies to investigate the correlation between wound-remodeling MMP expression and wound healing. Methods Thirty-eight wounds in 25 patients with traumatic extremity combat wounds were prospectively studied. Surgical debridement with vacuum-assisted closure (VAC) device application was repeated every 48 to 72h until surgical wound closure. Wound effluent and patient serum were collected at each wound debridement and analyzed for five matrix metalloproteinases using the Luminex multiplex system; Millipore Corp, Billerica, MA. The primary outcome was wound healing within 30 d of definitive wound closure. Impairment was defined as delayed wound closure (>21 d from injury) or wound dehiscence. MMP expression was compared between impaired and normal healing wounds. Results Elevated levels of serum MMP-2 and MMP-7 and reduced levels of effluent MMP3 were seen in impaired wounds ( n = 9) compared with wounds that healed ( n = 29; P
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2009.08.021