Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005

• Published in: Veterinary microbiology 2010-01, Vol.140 (1-2), p.56-62
• Main Authors: Swiatek, D.L., Palombo, E.A., Lee, A., Coventry, M.J., Britz, M.L., Kirkwood, C.D.
• Format: Article
• Language: English
• Subjects: Animals; Antigens, Viral - genetics; Australia; Biological and medical sciences; Bovine rotavirus; Capsid Proteins - genetics; Cattle; Cattle Diseases - virology; Diarrhea - veterinary; Diarrhea - virology; Feces - virology; Fundamental and applied biological sciences. Psychology; Genotype; Genotyping; Humans; Microbiology; Miscellaneous; Molecular Sequence Data; Phylogeny; Rotavirus - classification; Rotavirus - genetics; Rotavirus - isolation & purification; Rotavirus Infections - veterinary; Rotavirus Infections - virology; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Victoria; Virology; VP4; VP7
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2009.07.020

Bovine rotavirus (BRV) has been detected in both dairy and beef cattle herds worldwide. Stool samples collected from calves in the Gippsland region of Victoria, Australia were screened to determine the presence of BRV. A total of 100 faecal samples were collected from calves with and without diarrhoea across three farms during 2004 and 2005. Group A BRV was detected in 26% of faecal samples (22 from diarrheic calves and four from asymptomatic calves). Genotyping analysis of rotavirus positive samples indicated that G6P[5] was the most prevalent genotype (38.5%) followed by G6P[5+11] (15.4%). G10P[11] and G6+G10P[5] were each detected at a rate of 7.7%, and G6+G10P[11] was found in a single sample (3.8%). Seven samples (26.9%) could not be G and/or P typed. Thirty percent of the BRV positive samples were mixed infections, indicating that individual calves were co-infected with more than one strain of rotavirus. The G6P[5] strains exhibited high VP7 identity (>97% amino acid identity) with B-60, a G6 strain identified in Victorian calves during 1988. A G10P[11] isolate was closely related (>97% amino acid identity in VP7 and VP4 proteins) to a Victorian G10P[11] strain (B-11) also identified during 1988. This study demonstrates that BRV is a contributing pathogen to diarrhoeal disease in Victorian calves, with sequence analysis suggesting long-term conservation of the VP7 protein over a 16-year period.