Ru(II) Glycodendrimers as Probes to Study Lectin−Carbohydrate Interactions and Electrochemically Measure Monosaccharide and Oligosaccharide Concentrations

We describe a novel platform on which to study carbohydrate−protein interactions based on ruthenium(II) glycodendrimers as optical and electrochemical probes. Using the prototypical concanavalin A (ConA)−mannose lectin−carbohydrate interaction as an example, oligosaccharide concentrations were elect...

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Bibliographic Details
Published in:Langmuir 2010-02, Vol.26 (3), p.1520-1523
Main Authors: Kikkeri, Raghavendra, Kamena, Faustin, Gupta, Tarkeshwar, Hossain, Laila H, Boonyarattanakalin, Siwarutt, Gorodyska, Ganna, Beurer, Eva, Coullerez, Géraldine, Textor, Marcus, Seeberger, Peter H
Format: Article
Language:English
Subjects:
2,2'-Dipyridyl - chemistry
Carbohydrate Metabolism
Chemistry
Colloidal state and disperse state
Concanavalin A - metabolism
Dendrimers - chemistry
Electrochemistry
Exact sciences and technology
Fluorescent Dyes - chemistry
General and physical chemistry
Gold - chemistry
Lectins - metabolism
Mannose - metabolism
Monosaccharides - chemistry
Oligosaccharides - chemistry
Protein Array Analysis
Ruthenium - chemistry
Surface physical chemistry
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Summary:We describe a novel platform on which to study carbohydrate−protein interactions based on ruthenium(II) glycodendrimers as optical and electrochemical probes. Using the prototypical concanavalin A (ConA)−mannose lectin−carbohydrate interaction as an example, oligosaccharide concentrations were electrochemically monitored. The displacement of the Ru(II) complex from lectin-functionalized gold surfaces was repeatedly regenerated. This new platform presents a method to monitor many different complex sugars in parallel.
ISSN:0743-7463
1520-5827
DOI:10.1021/la9038792