Inhibitory Effects of Cationic Hybrid Liposomes on the Growth of Human Renal Cell Carcinoma

Cationic hybrid liposomes (HL) can be prepared by simply ultrasonicating a mixture of L-alpha-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene (21)dodecyl ether (C(12)(EO)(21)) and O,O'-ditetradecanoyl-N-(alpha-trimethylammonioacetyl) diethanolamine chloride (2C(14)ECl) in a buffer soluti...

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Bibliographic Details
Published in:Anticancer research 2010-02, Vol.30 (2), p.327-337
Main Authors: UMEBAYASHI, Masayo, MAKIZONO, Taku, ICHIHARA, Hideaki, MATSUMOTO, Yoko, UEOKA, Ryuichi
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Biological and medical sciences
Blotting, Western
Carcinoma, Renal Cell - drug therapy
Carcinoma, Renal Cell - metabolism
Carcinoma, Renal Cell - pathology
Caspases - metabolism
Cell Line, Tumor
Cell Proliferation - drug effects
Dimyristoylphosphatidylcholine - pharmacology
Female
Flow Cytometry
Hemolysis - drug effects
Humans
In Situ Nick-End Labeling
Kidney Neoplasms - drug therapy
Kidney Neoplasms - metabolism
Kidney Neoplasms - pathology
Kidneys
Liposomes
Medical sciences
Membrane Potential, Mitochondrial - drug effects
Mice
Mice, Inbred BALB C
Mice, Nude
Nephrology. Urinary tract diseases
Tumors
Tumors of the urinary system
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Summary:Cationic hybrid liposomes (HL) can be prepared by simply ultrasonicating a mixture of L-alpha-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene (21)dodecyl ether (C(12)(EO)(21)) and O,O'-ditetradecanoyl-N-(alpha-trimethylammonioacetyl) diethanolamine chloride (2C(14)ECl) in a buffer solution. The inhibitory effects of cationic HL containing 2C(14)ECl on the growth of OS-RC-2 human renal carcinoma cells in vitro and in vivo were examined. The 50% inhibitory concentration of cationic HL was remarkably reduced compared with that of DMPC liposomes. Induction of apoptosis by cationic HL in OS-RC-2 cells was verified on the basis of flow cytometric analysis and fluorescence microscopy in vitro. In addition, the effects on survival of cationic HL along with apoptosis in vivo were obtained using a mouse model of renal carcinoma. Chemotherapy with drug-free cationic HL for renal cell carcinoma was developed for the first time through the interaction between cationic HL and anionic surface region of tumor cell membranes, without any side-effects. The results obtained might be applied in chemotherapies used at present for patients with renal carcinoma.
ISSN:0250-7005
1791-7530