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role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats
The objective was to investigate the changes occurring in the activities of the enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in sheep and goat milk as a result of subclinical intramammary infections (IMI) and to evaluate the use of these enzyme...
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Published in: | Journal of dairy research 2010-02, Vol.77 (1), p.107-111 |
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creator | Katsoulos, Panagiotis D Christodoulopoulos, Georgios Minas, Anastasios Karatzia, Maria A Pourliotis, Konstantinos Kritas, Spyridon K |
description | The objective was to investigate the changes occurring in the activities of the enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in sheep and goat milk as a result of subclinical intramammary infections (IMI) and to evaluate the use of these enzymes for the diagnosis of subclinical IMI in dairy sheep and goats. A total of 206 samples of sheep milk and 162 samples of goat milk, obtained from equal udder halves, were used in the study. For each species they were divided into two groups: a no-infection group and a subclinical infection group. Activities of LDH, ALP and AST were significantly higher in the subclinical infection group than in the no-infection group (P |
doi_str_mv | 10.1017/S0022029909990410 |
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A total of 206 samples of sheep milk and 162 samples of goat milk, obtained from equal udder halves, were used in the study. For each species they were divided into two groups: a no-infection group and a subclinical infection group. Activities of LDH, ALP and AST were significantly higher in the subclinical infection group than in the no-infection group (P<0·05) in both sheep (LDH: 350·42±11·25 v. 120·91±4·41; ALP: 2773·43±105·18 v. 2189±94·24; AST: 29·57±0·74 v. 17·32±0·46) and goats (LDH: 354·07±13·33 v. 103·79±3·75; ALP: 311·13±25·74 v. 137·24±19·62; AST: 27·59±6·42 v. 15·87±0·45). The activity of LDH was identified as indicator for subclinical IMI in both sheep and goats. The optimum cut-off values for LDH activity, offering the highest diagnostic sensitivity (DSn) and diagnostic specificity (DSp), determined by receiver operating characteristic (ROC) analysis, were at 197 U/l, 185 U/l and 197 U/l for sheep, goats and both species, respectively. DSn for sheep, goats and both species at these cut-off values was 92·8%, 98·2% and 94·0%, whereas DSp was 95·4%, 96·3% and 96·3%, respectively. It was concluded that the determination of LDH activity in milk serum is a sensitive and reliable method for the detection of subclinical IMI in dairy sheep and goats.</description><identifier>ISSN: 0022-0299</identifier><identifier>EISSN: 1469-7629</identifier><identifier>DOI: 10.1017/S0022029909990410</identifier><identifier>PMID: 19919725</identifier><identifier>CODEN: JDRSAN</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>alkaline phosphatase ; Alkaline Phosphatase - metabolism ; Animal productions ; Animals ; Aspartate Aminotransferases - metabolism ; aspartate transaminase ; Bacterial Infections - diagnosis ; Bacterial Infections - enzymology ; Bacterial Infections - veterinary ; Biological and medical sciences ; Breast Diseases - enzymology ; Breast Diseases - microbiology ; Breast Diseases - veterinary ; dairy animals ; Dehydrogenase ; disease course ; disease diagnosis ; enzyme activity ; ewe milk ; ewes ; Female ; females ; Food industries ; Fundamental and applied biological sciences. Psychology ; Goat Diseases - diagnosis ; Goat Diseases - enzymology ; goat milk ; Goats ; L-Lactate Dehydrogenase - metabolism ; lactate dehydrogenase ; mastitis ; Milk ; Milk - enzymology ; Milk - microbiology ; milk analysis ; Milk and cheese industries. Ice creams ; milk composition ; Milk enzymes ; ROC Curve ; Sheep ; sheep and goats ; Sheep Diseases - diagnosis ; Sheep Diseases - enzymology ; subclinical intramammary infections ; subclinical mastitis ; Terrestrial animal productions ; Vertebrates</subject><ispartof>Journal of dairy research, 2010-02, Vol.77 (1), p.107-111</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © Proprietors of Journal of Dairy Research 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-90d3e17799b7cf0a01583beaf709e26e4197a5aa86db5035ac7f4a650141fcde3</citedby><cites>FETCH-LOGICAL-c419t-90d3e17799b7cf0a01583beaf709e26e4197a5aa86db5035ac7f4a650141fcde3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22486307$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19919725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katsoulos, Panagiotis D</creatorcontrib><creatorcontrib>Christodoulopoulos, Georgios</creatorcontrib><creatorcontrib>Minas, Anastasios</creatorcontrib><creatorcontrib>Karatzia, Maria A</creatorcontrib><creatorcontrib>Pourliotis, Konstantinos</creatorcontrib><creatorcontrib>Kritas, Spyridon K</creatorcontrib><title>role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats</title><title>Journal of dairy research</title><addtitle>Journal of Dairy Research</addtitle><description>The objective was to investigate the changes occurring in the activities of the enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in sheep and goat milk as a result of subclinical intramammary infections (IMI) and to evaluate the use of these enzymes for the diagnosis of subclinical IMI in dairy sheep and goats. A total of 206 samples of sheep milk and 162 samples of goat milk, obtained from equal udder halves, were used in the study. For each species they were divided into two groups: a no-infection group and a subclinical infection group. Activities of LDH, ALP and AST were significantly higher in the subclinical infection group than in the no-infection group (P<0·05) in both sheep (LDH: 350·42±11·25 v. 120·91±4·41; ALP: 2773·43±105·18 v. 2189±94·24; AST: 29·57±0·74 v. 17·32±0·46) and goats (LDH: 354·07±13·33 v. 103·79±3·75; ALP: 311·13±25·74 v. 137·24±19·62; AST: 27·59±6·42 v. 15·87±0·45). The activity of LDH was identified as indicator for subclinical IMI in both sheep and goats. The optimum cut-off values for LDH activity, offering the highest diagnostic sensitivity (DSn) and diagnostic specificity (DSp), determined by receiver operating characteristic (ROC) analysis, were at 197 U/l, 185 U/l and 197 U/l for sheep, goats and both species, respectively. DSn for sheep, goats and both species at these cut-off values was 92·8%, 98·2% and 94·0%, whereas DSp was 95·4%, 96·3% and 96·3%, respectively. It was concluded that the determination of LDH activity in milk serum is a sensitive and reliable method for the detection of subclinical IMI in dairy sheep and goats.</description><subject>alkaline phosphatase</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Aspartate Aminotransferases - metabolism</subject><subject>aspartate transaminase</subject><subject>Bacterial Infections - diagnosis</subject><subject>Bacterial Infections - enzymology</subject><subject>Bacterial Infections - veterinary</subject><subject>Biological and medical sciences</subject><subject>Breast Diseases - enzymology</subject><subject>Breast Diseases - microbiology</subject><subject>Breast Diseases - veterinary</subject><subject>dairy animals</subject><subject>Dehydrogenase</subject><subject>disease course</subject><subject>disease diagnosis</subject><subject>enzyme activity</subject><subject>ewe milk</subject><subject>ewes</subject><subject>Female</subject><subject>females</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Goat Diseases - diagnosis</subject><subject>Goat Diseases - enzymology</subject><subject>goat milk</subject><subject>Goats</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>lactate dehydrogenase</subject><subject>mastitis</subject><subject>Milk</subject><subject>Milk - enzymology</subject><subject>Milk - microbiology</subject><subject>milk analysis</subject><subject>Milk and cheese industries. Ice creams</subject><subject>milk composition</subject><subject>Milk enzymes</subject><subject>ROC Curve</subject><subject>Sheep</subject><subject>sheep and goats</subject><subject>Sheep Diseases - diagnosis</subject><subject>Sheep Diseases - enzymology</subject><subject>subclinical intramammary infections</subject><subject>subclinical mastitis</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><issn>0022-0299</issn><issn>1469-7629</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNplkt1u1DAQhSMEotvCA3ADERLihoB_kji-pCvaIlVCqO21NeuMd90mcfAkEn0eXrROd1UkuLAsz_nO8c84y95w9pkzrr5cMSYEE1oznUbJ2bNsxctaF6oW-nm2WuRi0Y-yY6Jbxrhkun6ZHXGtuVaiWmV_YugwDy7vwE4wYd7i7r6NYYsDEH7KobuDzg-Yj7tA4w6mVM1haHOgEeKjA3o_hCnCQA7jIvshn3YpycN2CORpiad5Y1OOt9AlPdE99D3E-7RwaCcfBlp8LfhUox3i-LjLNsBEr7IXDjrC14f5JLs5-3a9viguf5x_X3-9LGzJ9VRo1krkSmm9UdYxYLxq5AbBKaZR1JggBRVAU7ebiskKrHIl1BXjJXe2RXmSfdznjjH8mpEm03uy2HUwYJjJKCkbVilVJvL9P-RtmOOQDmeEaFhTVbJJEN9DNgaiiM6M0S93NpyZpX_mv_4lz9tD8Lzpsf3rODQsAR8OAFB6S5ee3Xp64oQom1oylbhiz3ma8PeTDvHO1EqqytTnP015dsqv-cXanCb-3Z53EAxsY8q8uRLLh-FKN40s5QNSfL3T</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Katsoulos, Panagiotis D</creator><creator>Christodoulopoulos, Georgios</creator><creator>Minas, Anastasios</creator><creator>Karatzia, Maria A</creator><creator>Pourliotis, Konstantinos</creator><creator>Kritas, Spyridon K</creator><general>Cambridge University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>7X8</scope></search><sort><creationdate>20100201</creationdate><title>role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats</title><author>Katsoulos, Panagiotis D ; Christodoulopoulos, Georgios ; Minas, Anastasios ; Karatzia, Maria A ; Pourliotis, Konstantinos ; Kritas, Spyridon K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-90d3e17799b7cf0a01583beaf709e26e4197a5aa86db5035ac7f4a650141fcde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>alkaline phosphatase</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Aspartate Aminotransferases - metabolism</topic><topic>aspartate transaminase</topic><topic>Bacterial Infections - diagnosis</topic><topic>Bacterial Infections - enzymology</topic><topic>Bacterial Infections - veterinary</topic><topic>Biological and medical sciences</topic><topic>Breast Diseases - enzymology</topic><topic>Breast Diseases - microbiology</topic><topic>Breast Diseases - veterinary</topic><topic>dairy animals</topic><topic>Dehydrogenase</topic><topic>disease course</topic><topic>disease diagnosis</topic><topic>enzyme activity</topic><topic>ewe milk</topic><topic>ewes</topic><topic>Female</topic><topic>females</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Goat Diseases - diagnosis</topic><topic>Goat Diseases - enzymology</topic><topic>goat milk</topic><topic>Goats</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>lactate dehydrogenase</topic><topic>mastitis</topic><topic>Milk</topic><topic>Milk - enzymology</topic><topic>Milk - microbiology</topic><topic>milk analysis</topic><topic>Milk and cheese industries. Ice creams</topic><topic>milk composition</topic><topic>Milk enzymes</topic><topic>ROC Curve</topic><topic>Sheep</topic><topic>sheep and goats</topic><topic>Sheep Diseases - diagnosis</topic><topic>Sheep Diseases - enzymology</topic><topic>subclinical intramammary infections</topic><topic>subclinical mastitis</topic><topic>Terrestrial animal productions</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katsoulos, Panagiotis D</creatorcontrib><creatorcontrib>Christodoulopoulos, Georgios</creatorcontrib><creatorcontrib>Minas, Anastasios</creatorcontrib><creatorcontrib>Karatzia, Maria A</creatorcontrib><creatorcontrib>Pourliotis, Konstantinos</creatorcontrib><creatorcontrib>Kritas, Spyridon K</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katsoulos, Panagiotis D</au><au>Christodoulopoulos, Georgios</au><au>Minas, Anastasios</au><au>Karatzia, Maria A</au><au>Pourliotis, Konstantinos</au><au>Kritas, Spyridon K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats</atitle><jtitle>Journal of dairy research</jtitle><addtitle>Journal of Dairy Research</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>77</volume><issue>1</issue><spage>107</spage><epage>111</epage><pages>107-111</pages><issn>0022-0299</issn><eissn>1469-7629</eissn><coden>JDRSAN</coden><abstract>The objective was to investigate the changes occurring in the activities of the enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in sheep and goat milk as a result of subclinical intramammary infections (IMI) and to evaluate the use of these enzymes for the diagnosis of subclinical IMI in dairy sheep and goats. A total of 206 samples of sheep milk and 162 samples of goat milk, obtained from equal udder halves, were used in the study. For each species they were divided into two groups: a no-infection group and a subclinical infection group. Activities of LDH, ALP and AST were significantly higher in the subclinical infection group than in the no-infection group (P<0·05) in both sheep (LDH: 350·42±11·25 v. 120·91±4·41; ALP: 2773·43±105·18 v. 2189±94·24; AST: 29·57±0·74 v. 17·32±0·46) and goats (LDH: 354·07±13·33 v. 103·79±3·75; ALP: 311·13±25·74 v. 137·24±19·62; AST: 27·59±6·42 v. 15·87±0·45). The activity of LDH was identified as indicator for subclinical IMI in both sheep and goats. The optimum cut-off values for LDH activity, offering the highest diagnostic sensitivity (DSn) and diagnostic specificity (DSp), determined by receiver operating characteristic (ROC) analysis, were at 197 U/l, 185 U/l and 197 U/l for sheep, goats and both species, respectively. DSn for sheep, goats and both species at these cut-off values was 92·8%, 98·2% and 94·0%, whereas DSp was 95·4%, 96·3% and 96·3%, respectively. It was concluded that the determination of LDH activity in milk serum is a sensitive and reliable method for the detection of subclinical IMI in dairy sheep and goats.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>19919725</pmid><doi>10.1017/S0022029909990410</doi><tpages>5</tpages></addata></record> |
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subjects | alkaline phosphatase Alkaline Phosphatase - metabolism Animal productions Animals Aspartate Aminotransferases - metabolism aspartate transaminase Bacterial Infections - diagnosis Bacterial Infections - enzymology Bacterial Infections - veterinary Biological and medical sciences Breast Diseases - enzymology Breast Diseases - microbiology Breast Diseases - veterinary dairy animals Dehydrogenase disease course disease diagnosis enzyme activity ewe milk ewes Female females Food industries Fundamental and applied biological sciences. Psychology Goat Diseases - diagnosis Goat Diseases - enzymology goat milk Goats L-Lactate Dehydrogenase - metabolism lactate dehydrogenase mastitis Milk Milk - enzymology Milk - microbiology milk analysis Milk and cheese industries. Ice creams milk composition Milk enzymes ROC Curve Sheep sheep and goats Sheep Diseases - diagnosis Sheep Diseases - enzymology subclinical intramammary infections subclinical mastitis Terrestrial animal productions Vertebrates |
title | role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats |
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