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role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats

The objective was to investigate the changes occurring in the activities of the enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in sheep and goat milk as a result of subclinical intramammary infections (IMI) and to evaluate the use of these enzyme...

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Published in:Journal of dairy research 2010-02, Vol.77 (1), p.107-111
Main Authors: Katsoulos, Panagiotis D, Christodoulopoulos, Georgios, Minas, Anastasios, Karatzia, Maria A, Pourliotis, Konstantinos, Kritas, Spyridon K
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container_title Journal of dairy research
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description The objective was to investigate the changes occurring in the activities of the enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in sheep and goat milk as a result of subclinical intramammary infections (IMI) and to evaluate the use of these enzymes for the diagnosis of subclinical IMI in dairy sheep and goats. A total of 206 samples of sheep milk and 162 samples of goat milk, obtained from equal udder halves, were used in the study. For each species they were divided into two groups: a no-infection group and a subclinical infection group. Activities of LDH, ALP and AST were significantly higher in the subclinical infection group than in the no-infection group (P
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A total of 206 samples of sheep milk and 162 samples of goat milk, obtained from equal udder halves, were used in the study. For each species they were divided into two groups: a no-infection group and a subclinical infection group. Activities of LDH, ALP and AST were significantly higher in the subclinical infection group than in the no-infection group (P&lt;0·05) in both sheep (LDH: 350·42±11·25 v. 120·91±4·41; ALP: 2773·43±105·18 v. 2189±94·24; AST: 29·57±0·74 v. 17·32±0·46) and goats (LDH: 354·07±13·33 v. 103·79±3·75; ALP: 311·13±25·74 v. 137·24±19·62; AST: 27·59±6·42 v. 15·87±0·45). The activity of LDH was identified as indicator for subclinical IMI in both sheep and goats. The optimum cut-off values for LDH activity, offering the highest diagnostic sensitivity (DSn) and diagnostic specificity (DSp), determined by receiver operating characteristic (ROC) analysis, were at 197 U/l, 185 U/l and 197 U/l for sheep, goats and both species, respectively. DSn for sheep, goats and both species at these cut-off values was 92·8%, 98·2% and 94·0%, whereas DSp was 95·4%, 96·3% and 96·3%, respectively. It was concluded that the determination of LDH activity in milk serum is a sensitive and reliable method for the detection of subclinical IMI in dairy sheep and goats.</description><identifier>ISSN: 0022-0299</identifier><identifier>EISSN: 1469-7629</identifier><identifier>DOI: 10.1017/S0022029909990410</identifier><identifier>PMID: 19919725</identifier><identifier>CODEN: JDRSAN</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>alkaline phosphatase ; Alkaline Phosphatase - metabolism ; Animal productions ; Animals ; Aspartate Aminotransferases - metabolism ; aspartate transaminase ; Bacterial Infections - diagnosis ; Bacterial Infections - enzymology ; Bacterial Infections - veterinary ; Biological and medical sciences ; Breast Diseases - enzymology ; Breast Diseases - microbiology ; Breast Diseases - veterinary ; dairy animals ; Dehydrogenase ; disease course ; disease diagnosis ; enzyme activity ; ewe milk ; ewes ; Female ; females ; Food industries ; Fundamental and applied biological sciences. Psychology ; Goat Diseases - diagnosis ; Goat Diseases - enzymology ; goat milk ; Goats ; L-Lactate Dehydrogenase - metabolism ; lactate dehydrogenase ; mastitis ; Milk ; Milk - enzymology ; Milk - microbiology ; milk analysis ; Milk and cheese industries. Ice creams ; milk composition ; Milk enzymes ; ROC Curve ; Sheep ; sheep and goats ; Sheep Diseases - diagnosis ; Sheep Diseases - enzymology ; subclinical intramammary infections ; subclinical mastitis ; Terrestrial animal productions ; Vertebrates</subject><ispartof>Journal of dairy research, 2010-02, Vol.77 (1), p.107-111</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © Proprietors of Journal of Dairy Research 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-90d3e17799b7cf0a01583beaf709e26e4197a5aa86db5035ac7f4a650141fcde3</citedby><cites>FETCH-LOGICAL-c419t-90d3e17799b7cf0a01583beaf709e26e4197a5aa86db5035ac7f4a650141fcde3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=22486307$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19919725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katsoulos, Panagiotis D</creatorcontrib><creatorcontrib>Christodoulopoulos, Georgios</creatorcontrib><creatorcontrib>Minas, Anastasios</creatorcontrib><creatorcontrib>Karatzia, Maria A</creatorcontrib><creatorcontrib>Pourliotis, Konstantinos</creatorcontrib><creatorcontrib>Kritas, Spyridon K</creatorcontrib><title>role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats</title><title>Journal of dairy research</title><addtitle>Journal of Dairy Research</addtitle><description>The objective was to investigate the changes occurring in the activities of the enzymes lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in sheep and goat milk as a result of subclinical intramammary infections (IMI) and to evaluate the use of these enzymes for the diagnosis of subclinical IMI in dairy sheep and goats. A total of 206 samples of sheep milk and 162 samples of goat milk, obtained from equal udder halves, were used in the study. For each species they were divided into two groups: a no-infection group and a subclinical infection group. Activities of LDH, ALP and AST were significantly higher in the subclinical infection group than in the no-infection group (P&lt;0·05) in both sheep (LDH: 350·42±11·25 v. 120·91±4·41; ALP: 2773·43±105·18 v. 2189±94·24; AST: 29·57±0·74 v. 17·32±0·46) and goats (LDH: 354·07±13·33 v. 103·79±3·75; ALP: 311·13±25·74 v. 137·24±19·62; AST: 27·59±6·42 v. 15·87±0·45). The activity of LDH was identified as indicator for subclinical IMI in both sheep and goats. The optimum cut-off values for LDH activity, offering the highest diagnostic sensitivity (DSn) and diagnostic specificity (DSp), determined by receiver operating characteristic (ROC) analysis, were at 197 U/l, 185 U/l and 197 U/l for sheep, goats and both species, respectively. DSn for sheep, goats and both species at these cut-off values was 92·8%, 98·2% and 94·0%, whereas DSp was 95·4%, 96·3% and 96·3%, respectively. It was concluded that the determination of LDH activity in milk serum is a sensitive and reliable method for the detection of subclinical IMI in dairy sheep and goats.</description><subject>alkaline phosphatase</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animal productions</subject><subject>Animals</subject><subject>Aspartate Aminotransferases - metabolism</subject><subject>aspartate transaminase</subject><subject>Bacterial Infections - diagnosis</subject><subject>Bacterial Infections - enzymology</subject><subject>Bacterial Infections - veterinary</subject><subject>Biological and medical sciences</subject><subject>Breast Diseases - enzymology</subject><subject>Breast Diseases - microbiology</subject><subject>Breast Diseases - veterinary</subject><subject>dairy animals</subject><subject>Dehydrogenase</subject><subject>disease course</subject><subject>disease diagnosis</subject><subject>enzyme activity</subject><subject>ewe milk</subject><subject>ewes</subject><subject>Female</subject><subject>females</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Goat Diseases - diagnosis</subject><subject>Goat Diseases - enzymology</subject><subject>goat milk</subject><subject>Goats</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>lactate dehydrogenase</subject><subject>mastitis</subject><subject>Milk</subject><subject>Milk - enzymology</subject><subject>Milk - microbiology</subject><subject>milk analysis</subject><subject>Milk and cheese industries. Ice creams</subject><subject>milk composition</subject><subject>Milk enzymes</subject><subject>ROC Curve</subject><subject>Sheep</subject><subject>sheep and goats</subject><subject>Sheep Diseases - diagnosis</subject><subject>Sheep Diseases - enzymology</subject><subject>subclinical intramammary infections</subject><subject>subclinical mastitis</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><issn>0022-0299</issn><issn>1469-7629</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNplkt1u1DAQhSMEotvCA3ADERLihoB_kji-pCvaIlVCqO21NeuMd90mcfAkEn0eXrROd1UkuLAsz_nO8c84y95w9pkzrr5cMSYEE1oznUbJ2bNsxctaF6oW-nm2WuRi0Y-yY6Jbxrhkun6ZHXGtuVaiWmV_YugwDy7vwE4wYd7i7r6NYYsDEH7KobuDzg-Yj7tA4w6mVM1haHOgEeKjA3o_hCnCQA7jIvshn3YpycN2CORpiad5Y1OOt9AlPdE99D3E-7RwaCcfBlp8LfhUox3i-LjLNsBEr7IXDjrC14f5JLs5-3a9viguf5x_X3-9LGzJ9VRo1krkSmm9UdYxYLxq5AbBKaZR1JggBRVAU7ebiskKrHIl1BXjJXe2RXmSfdznjjH8mpEm03uy2HUwYJjJKCkbVilVJvL9P-RtmOOQDmeEaFhTVbJJEN9DNgaiiM6M0S93NpyZpX_mv_4lz9tD8Lzpsf3rODQsAR8OAFB6S5ee3Xp64oQom1oylbhiz3ma8PeTDvHO1EqqytTnP015dsqv-cXanCb-3Z53EAxsY8q8uRLLh-FKN40s5QNSfL3T</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Katsoulos, Panagiotis D</creator><creator>Christodoulopoulos, Georgios</creator><creator>Minas, Anastasios</creator><creator>Karatzia, Maria A</creator><creator>Pourliotis, Konstantinos</creator><creator>Kritas, Spyridon K</creator><general>Cambridge University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>7X8</scope></search><sort><creationdate>20100201</creationdate><title>role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats</title><author>Katsoulos, Panagiotis D ; Christodoulopoulos, Georgios ; Minas, Anastasios ; Karatzia, Maria A ; Pourliotis, Konstantinos ; Kritas, Spyridon K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-90d3e17799b7cf0a01583beaf709e26e4197a5aa86db5035ac7f4a650141fcde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>alkaline phosphatase</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animal productions</topic><topic>Animals</topic><topic>Aspartate Aminotransferases - metabolism</topic><topic>aspartate transaminase</topic><topic>Bacterial Infections - diagnosis</topic><topic>Bacterial Infections - enzymology</topic><topic>Bacterial Infections - veterinary</topic><topic>Biological and medical sciences</topic><topic>Breast Diseases - enzymology</topic><topic>Breast Diseases - microbiology</topic><topic>Breast Diseases - veterinary</topic><topic>dairy animals</topic><topic>Dehydrogenase</topic><topic>disease course</topic><topic>disease diagnosis</topic><topic>enzyme activity</topic><topic>ewe milk</topic><topic>ewes</topic><topic>Female</topic><topic>females</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. 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A total of 206 samples of sheep milk and 162 samples of goat milk, obtained from equal udder halves, were used in the study. For each species they were divided into two groups: a no-infection group and a subclinical infection group. Activities of LDH, ALP and AST were significantly higher in the subclinical infection group than in the no-infection group (P&lt;0·05) in both sheep (LDH: 350·42±11·25 v. 120·91±4·41; ALP: 2773·43±105·18 v. 2189±94·24; AST: 29·57±0·74 v. 17·32±0·46) and goats (LDH: 354·07±13·33 v. 103·79±3·75; ALP: 311·13±25·74 v. 137·24±19·62; AST: 27·59±6·42 v. 15·87±0·45). The activity of LDH was identified as indicator for subclinical IMI in both sheep and goats. The optimum cut-off values for LDH activity, offering the highest diagnostic sensitivity (DSn) and diagnostic specificity (DSp), determined by receiver operating characteristic (ROC) analysis, were at 197 U/l, 185 U/l and 197 U/l for sheep, goats and both species, respectively. DSn for sheep, goats and both species at these cut-off values was 92·8%, 98·2% and 94·0%, whereas DSp was 95·4%, 96·3% and 96·3%, respectively. It was concluded that the determination of LDH activity in milk serum is a sensitive and reliable method for the detection of subclinical IMI in dairy sheep and goats.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>19919725</pmid><doi>10.1017/S0022029909990410</doi><tpages>5</tpages></addata></record>
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subjects alkaline phosphatase
Alkaline Phosphatase - metabolism
Animal productions
Animals
Aspartate Aminotransferases - metabolism
aspartate transaminase
Bacterial Infections - diagnosis
Bacterial Infections - enzymology
Bacterial Infections - veterinary
Biological and medical sciences
Breast Diseases - enzymology
Breast Diseases - microbiology
Breast Diseases - veterinary
dairy animals
Dehydrogenase
disease course
disease diagnosis
enzyme activity
ewe milk
ewes
Female
females
Food industries
Fundamental and applied biological sciences. Psychology
Goat Diseases - diagnosis
Goat Diseases - enzymology
goat milk
Goats
L-Lactate Dehydrogenase - metabolism
lactate dehydrogenase
mastitis
Milk
Milk - enzymology
Milk - microbiology
milk analysis
Milk and cheese industries. Ice creams
milk composition
Milk enzymes
ROC Curve
Sheep
sheep and goats
Sheep Diseases - diagnosis
Sheep Diseases - enzymology
subclinical intramammary infections
subclinical mastitis
Terrestrial animal productions
Vertebrates
title role of lactate dehydrogenase, alkaline phosphatase and aspartate aminotransferase in the diagnosis of subclinical intramammary infections in dairy sheep and goats
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