Serum testosterone bioassay evaluation in a large male cohort

Summary Objective  To assess if a cell‐based readout of androgen action in serum demonstrates a closer association with recognized classical parameters of androgen action in men than current measures of serum testosterone (T). Design  To develop, validate and utilize a mammalian cell‐based assay to...

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Bibliographic Details
Published in:Clinical endocrinology (Oxford) 2010-01, Vol.72 (1), p.87-98
Main Authors: Need, Eleanor F., O'Loughlin, Peter D., Armstrong, David T., Haren, Matthew T., Martin, Sean A., Tilley, Wayne D., Wittert, Gary A., Buchanan, Grant
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Animals
Biological and medical sciences
Biological Assay - methods
Biological Assay - standards
Cells, Cultured
Cercopithecus aethiops
CHO Cells
Cohort Studies
COS Cells
Cricetinae
Cricetulus
Diagnostic Techniques, Endocrine - standards
Endocrinopathies
Fundamental and applied biological sciences. Psychology
Humans
Male
Medical sciences
Middle Aged
Miscellaneous
Public health. Hygiene
Public health. Hygiene-occupational medicine
Testosterone - analysis
Testosterone - blood
Testosterone - standards
Vertebrates: endocrinology
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Summary:Summary Objective  To assess if a cell‐based readout of androgen action in serum demonstrates a closer association with recognized classical parameters of androgen action in men than current measures of serum testosterone (T). Design  To develop, validate and utilize a mammalian cell‐based assay to measure specifically bioactive T and determine if this measure is a physiologically relevant fraction of serum T. Measurements and participants  We have developed a specific serum T bioassay using human prostate cancer cells. A rapid 5‐min exposure to 100% serum followed by serum withdrawal confers specificity of the assay to serum T and provides sufficient sensitivity to measure T in male serum samples. Matrix effects were experimentally discounted as a confounding issue. A total of 960 male serum samples from the Florey Adelaide Male Ageing Study (FAMAS) with previous comprehensive cohort data and serum measurements were utilized. Results  Bioassay T measurement in the 960 FAMAS serum samples returned a median of 10·7 nmol/l (1·7–45·4), and was most closely related to immunoassayed total T, but not immunoassayed bioavailable T or calculated free T. Immunoassayed total T demonstrated a positive association with isometric grip‐strength (R2 = 0·127, P 
ISSN:0300-0664
1365-2265
DOI:10.1111/j.1365-2265.2009.03595.x