Progress in phage display: evolution of the technique and its application

Phage display, the presentation of (poly)peptides as fusions to capsid proteins on the surface of bacterial viruses, celebrates its 25th birthday in 2010. The technique, coupled with in vitro selection, enables rapid identification and optimization of proteins based on their structural or functional...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 2010-03, Vol.67 (5), p.749-767
Main Author: Bratkovic, Tomaz
Format: Article
Language:English
Subjects:
Animals
Biopharmaceutics - methods
Biopharmaceutics - trends
Cloning, Molecular - methods
Combinatorial Chemistry Techniques - methods
Combinatorial Chemistry Techniques - trends
Efficiency
High-Throughput Screening Assays - methods
High-Throughput Screening Assays - trends
Humans
Models, Biological
Peptide Library
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Summary:Phage display, the presentation of (poly)peptides as fusions to capsid proteins on the surface of bacterial viruses, celebrates its 25th birthday in 2010. The technique, coupled with in vitro selection, enables rapid identification and optimization of proteins based on their structural or functional properties. In the last two decades, it has advanced tremendously and has become widely accepted by the scientific community. This by no means exhaustive review aims to inform the reader of the key modifications in phage display. Novel display formats, innovative library designs and screening strategies are discussed. I will also briefly review some recent uses of the technology to illustrate its incredible versatility.
ISSN:1420-9071