Osteoblast growth and function in porous poly ε-caprolactone matrices for bone repair: a preliminary study

• Published in: Biomaterials 2003-09, Vol.24 (21), p.3815-3824
• Main Authors: Ciapetti, G., Ambrosio, L., Savarino, L., Granchi, D., Cenni, E., Baldini, N., Pagani, S., Guizzardi, S., Causa, F., Giunti, A.
• Format: Article
• Language: English
• Subjects: Biocompatible Materials - chemistry; Biodegradation; Bone Regeneration; Bone Substitutes; Caproates - chemistry; Cell Line; Cell Survival; Durapatite - chemistry; Humans; Hydroxyapatite; Lactones - chemistry; Microscopy, Electron, Scanning; Osteoblasts; Osteoblasts - chemistry; Osteoblasts - physiology; Osteogenesis; Poly ε-caprolactone; Polyesters - chemistry; Polymers; Polymers - chemistry; Time Factors; Wound Healing
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(03)00263-1

Current methods for the replacement of skeletal tissue involve the use of autografts, allografts and, recently, synthetic substitutes, which provide a proper amount of material to repair large bone defects. Engineered bone seems a promising approach, but a number of variables have to be set prior to any clinical application. In this study, four different poly caprolactone-based polymers (PCL) were prepared and tested in vitro using osteoblast-like Saos-2 cells. Differences among three-dimensional polymers include porosity, addition of hydroxyapatite (HA) particles, and treatment with simulated body fluid. Biochemical parameters to assess cell/material interactions include viability, growth, alkaline phosphatase release, and mineralization of osteoblastic cells seeded onto three-dimensional samples, while their morphology was observed using light microscopy and SEM. Preliminary results show that the polymers, though degrading in the medium, have a positive interaction with cells, as they support cell growth and functions. In the short-term culture (3–7 days) of Saos-2 on polymers, little differences were found among PCL samples, with the presence of HA moderately improving the number of cells onto the surfaces. In the long term (3–4 weeks), it was found that the HA-added polymers obtained the best colonization by cells, and more mineral formation was observed after coating with SBF. It can be concluded that PCL is a promising material for three-dimensional scaffold for bone formation, and the presence of bone-like components improves osteoblast activity.