A subunit vaccine against the adenovirus egg-drop syndrome using part of its fiber protein

• Published in: Vaccine 2003-06, Vol.21 (21), p.2761-2766
• Main Authors: Fingerut, E, Gutter, B, Gallili, G, Michael, A, Pitcovski, J
• Format: Article
• Language: English
• Subjects: Adenoviridae Infections - immunology; Adenoviridae Infections - prevention & control; Adenoviridae Infections - veterinary; Adenovirus; Adenoviruses; Amino acids; Animals; Biological and medical sciences; Capsid Proteins - biosynthesis; Capsid Proteins - immunology; Deoxyribonucleic acid; Disease; DNA; DNA - genetics; Ducks; E coli; Egg Shell - pathology; Egg-drop syndrome; Eggs; Escherichia coli - metabolism; Fundamental and applied biological sciences. Psychology; Hemagglutination inhibition; Microbiology; Neutralization; Polypeptides; Poultry; Poultry Diseases - immunology; Poultry Diseases - prevention & control; Proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - immunology; Serum neutralization; Subunit vaccine; Vaccination; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Subunit - immunology; Viral Vaccines - immunology; Virology; Viruses
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(03)00117-8

In this study, the effectiveness of antibodies against the hexon, fiber or a fiber fragment of an avian adenovirus egg-drop syndrome (EDS), in neutralizing the virus was tested. The fiber protein is responsible for binding the virus to the target cell. The fiber fragment knob-s comprises the carboxy-terminal knob domain and 34 amino acids of the immediately adjacent shaft domain of the adenovirus fiber protein. The hexon, fiber capsid protein and knob-s were produced in E. coli and injected into chickens. Antibodies that were produced against the whole fiber protein showed some hemagglutination inhibition (HI) activity. Antibodies produced against the knob-s protein showed HI activity and serum neutralization (SN) activity similar to the positive control—whole virus vaccine. We assume that production of only part of the fiber enables the protein produced in E. coli to fold correctly. Antibodies produced against the hexon protein showed no SN activity. In summary, knob-s induced SN and HI antibodies against EDS virus at a rate similar to the whole virus and were significantly more efficient than the full-length fiber. The recombinant knob-s protein may be used as a vaccine against pathogenic adenovirus infections.