Iothalamate Quantification by Tandem Mass Spectrometry to Measure Glomerular Filtration Rate

Glomerular filtration rate (GFR) can be determined by measuring renal clearance of the radiocontrast agent iothalamate. Current analytic methods for quantifying iothalamate concentrations in plasma and urine using liquid chromatography or capillary electrophoresis have limitations such as long analy...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2010-04, Vol.56 (4), p.568-574
Main Authors: Seegmiller, Jesse C, Burns, Bradley E, Fauq, Abdul H, Mukhtar, Naveen, Lieske, John C, Larson, Timothy S
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Centrifugation
Chromatography, Liquid
Clinical medicine
Electrophoresis
Electrophoresis, Capillary
Fundamental and applied biological sciences. Psychology
Glomerular Filtration Rate
Humans
Investigative techniques, diagnostic techniques (general aspects)
Iothalamic Acid - analysis
Kidney diseases
Liquid chromatography
Mass spectrometry
Medical sciences
Methods
Molecular biophysics
Molecular weight
Mortality
Reproducibility of Results
Sensitivity and Specificity
Studies
Tandem Mass Spectrometry
Time Factors
Urine
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Summary:Glomerular filtration rate (GFR) can be determined by measuring renal clearance of the radiocontrast agent iothalamate. Current analytic methods for quantifying iothalamate concentrations in plasma and urine using liquid chromatography or capillary electrophoresis have limitations such as long analysis times and susceptibility to interferences. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to overcome these limitations. Urine and plasma samples were deproteinized using acetonitrile and centrifugation. The supernatant was diluted in water and analyzed by LC-MS/MS using a water:methanol gradient. We monitored 4 multiple reaction monitoring transitions: m/z 614.8-487.0, 614.8-456.0, 614.8-361.1, and 614.8-177.1. We compared the results to those obtained via our standard capillary electrophoresis (CE-UV) on samples from 53 patients undergoing clinical GFR testing. Mean recovery was 90%-110% in both urine and plasma matrices. Imprecision was
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2009.133751