Colorimetric determination of some penicillins and cephalosporins with 2-nitrophenylhydrazine hydrochloride

A simple and sensitive calorimetric method for the determination of some penicillins and cephalosporins is presented. The method is based on reaction with 2-nitrophenylhydrazine hydrochloride in presence of dicyclohexylcarbodi-imide and pyridine. The violet colour of the resulting acid hydrazide is...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 1989-12, Vol.36 (12), p.1253-1257
Main Authors: Korany, Mohamed A., Abdel-Hay, Mohamed H., Bedair, Mona M., Gazy, Azza A.
Format: Article
Language:English
Subjects:
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
General aspects
Medical sciences
Pharmacology. Drug treatments
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple and sensitive calorimetric method for the determination of some penicillins and cephalosporins is presented. The method is based on reaction with 2-nitrophenylhydrazine hydrochloride in presence of dicyclohexylcarbodi-imide and pyridine. The violet colour of the resulting acid hydrazide is measured at the appropriate wavelength. The method has been applied to determination of these antibiotics in bulk and dosage forms, with a coefficient of variation less than 2%.
ISSN:0039-9140
1873-3573
DOI:10.1016/0039-9140(89)80059-1