Simultaneous spectrophotometric determination of three food dyes by using the first derivative of ratio spectra

Ternary mixtures of colorants E-123 (Amaranth), E-124 (Ponceau 4R) and E-120 (Carminic acid) are resolved by using the first derivative ratio spectrum-zero crossing method without the need for any separation step. Calibration graphs were linear up to 64 mg l −1 of carminic acid, 32 mg l −1 of Amaran...

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Bibliographic Details
Published in:Talanta (Oxford) 1995-12, Vol.42 (12), p.2043-2051
Main Authors: Nevado, J.J.Berzas, Cabanillas, C.Guiberteau, Salcedo, A.M.Contento
Format: Article
Language:English
Subjects:
Biological and medical sciences
First derivative spectrophotometry
Food additives
Food dyes
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
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Summary:Ternary mixtures of colorants E-123 (Amaranth), E-124 (Ponceau 4R) and E-120 (Carminic acid) are resolved by using the first derivative ratio spectrum-zero crossing method without the need for any separation step. Calibration graphs were linear up to 64 mg l −1 of carminic acid, 32 mg l −1 of Amaranth and 32 mg l −1 of Ponceau 4R. The method was applied to different commercial food products and results concordant with high performance liquid chromatography were obtained.
ISSN:0039-9140
1873-3573
DOI:10.1016/0039-9140(95)01695-3