Determination of diphenhydramine by capillary electrophoresis with tris(2,2′-bipyridyl)ruthenium(II) electrochemiluminescence detection

Tris(2,2′-bipyridyl)ruthenium(II) electrochemiluminescence detection in a capillary electrophoresis separation system was used for the determination of diphenhydramine. In this study, platinum disk electrode (300 μm in diameter) was used as a working electrode and the influence of applied potential...

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Bibliographic Details
Published in:Talanta (Oxford) 2003-03, Vol.59 (3), p.453-459
Main Authors: Liu, Jifeng, Cao, Weidong, Yang, Xiurong, Wang, Erkang
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Capillary electrophoresis
Diphenhydramine
Electrochemiluminescence detection
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Rabbit plasma
Rabbit urine
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Summary:Tris(2,2′-bipyridyl)ruthenium(II) electrochemiluminescence detection in a capillary electrophoresis separation system was used for the determination of diphenhydramine. In this study, platinum disk electrode (300 μm in diameter) was used as a working electrode and the influence of applied potential and buffer conditions were investigated. Under optimal conditions: 1.2 V applied potential, pH 8.50, 15 kV separation voltage and 10 mmol l −1 running buffer, the calibration curve of diphenhydramine was linear over the range of 4×10 −8 to 1×10 −5 mol l −1. This technique gave satisfactory precision, and relative standard deviations of migration times and chemiluminescence peak intensities were less than 1 and 6%, respectively. The technique was applied to animal studies for determination of diphenhydramine extracted from rabbit plasma and urine samples, and the extraction efficiency were between 92 and 98.5%.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(02)00535-0