Chemiluminescent determination of pyridoxine hydrochloride in pharmaceutical samples using flow injection

A chemiluminescent method using flow injection is described for the determination of pyridoxine hydrochloride. Its detection limit, linearity and reproducibility were examined. The method is based on the enhancing effect of pyridoxine hydrochloride on the chemiluminescence generated by the oxidation...

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Bibliographic Details
Published in:Talanta (Oxford) 1998-04, Vol.45 (6), p.1131-1138
Main Authors: Alwarthan, Abdulrahman A., A. Aly, Fatima
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chemiluminescence determination
Dietary sources
Flow injection
General pharmacology
Luminol
Medical sciences
Pharmacology. Drug treatments
Pyridoxine·HCl
Tablet formulation
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Description
Summary:A chemiluminescent method using flow injection is described for the determination of pyridoxine hydrochloride. Its detection limit, linearity and reproducibility were examined. The method is based on the enhancing effect of pyridoxine hydrochloride on the chemiluminescence generated by the oxidation of luminol with hydrogen peroxide in aqueous potassium hydroxide and sodium oxalate. The proposed method is simple and inexpensive. The chemiluminescence intensity is a linear function of pyridoxine hydrochloride concentration over the range 10–250 μg ml −1 with a detection limit of 6 μg ml −1. The applicability of the method was demonstrated by the determination of pyridoxine hydrochloride in different tablet formulations and some dietary sources.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(97)00223-3