Spectrophotometric flow injection determination of l-ascorbic acid with a packed reactor containing ferric hydroxide

A flow injection system with spectrophotometric detection is proposed for determining l-ascorbic acid in pharmaceutical formulations. In this system a column containing Fe(OH) 3 immobilized in polyester resin (packed reactor) is inserted before the detector. Fe(III)-1,10-phenanthroline complex is re...

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Bibliographic Details
Published in:Talanta (Oxford) 1998-09, Vol.47 (1), p.11-18
Main Authors: Pereira, Airton Vicente, Fatibello-Filho, Orlando
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Ferric hydroxide
Flow injection spectrophotometry
General pharmacology
l-Ascorbic acid
Medical sciences
Packed reactor
Pharmaceutical formulations
Pharmacology. Drug treatments
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Description
Summary:A flow injection system with spectrophotometric detection is proposed for determining l-ascorbic acid in pharmaceutical formulations. In this system a column containing Fe(OH) 3 immobilized in polyester resin (packed reactor) is inserted before the detector. Fe(III)-1,10-phenanthroline complex is reduced by l–ascorbic acid to produce Fe(II)-1,10-phenanthroline complex which is monitored at 510 nm. Under the optimum analytical conditions, the linearity of the calibration equation for l-ascorbic acid ranged from 5.0×10 −6 to 6.0×10 −5 M of added amount. The detection limit was 5.0×10 −7 M and recoveries between 98.5–102.0% were obtained. No interference was observed from the common excipients of pharmaceutical formulations and other active substances such as acetylsalicylic acid, caffeine and thiamine.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(98)00047-2