Determination of selenium species in human urine by high performance liquid chromatography and inductively coupled plasma mass spectrometry

A method developed to determine organic and inorganic selenium species in human urine samples is presented in detail. After a simple sample treatment based on elimination of non-charged organic compounds, selenium species were separated by high performance liquid chromatography (HPLC) on a Spherisor...

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Bibliographic Details
Published in:Talanta (Oxford) 1999-08, Vol.50 (1), p.165-173
Main Authors: Quijano, M.Angeles, Gutiérrez, Ana Maria, Pérez-Conde, M.Concepción, Cámara, Carmen
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Gas, fumes
High performance liquid chromatography
Human urine
Inductively coupled plasma mass spectrometry
Medical sciences
Selenium speciation
Toxicology
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Summary:A method developed to determine organic and inorganic selenium species in human urine samples is presented in detail. After a simple sample treatment based on elimination of non-charged organic compounds, selenium species were separated by high performance liquid chromatography (HPLC) on a Spherisorb 5 ODS/AMINO column using two different chromatographic conditions: phosphate buffers at pH 2.8 and 6.0. Detection was carried out using an on-line inductively coupled plasma mass spectrometer (ICP-MS). Trimethylselenonium ion and two unknown selenium species in urine samples were found. Selenium species were shown to have stability problems, with the maximum allowed storage time of 1 week.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(99)00115-0