Investigation on the binding site in heparin by spectrophotometry

Heparin has a variety of biological activities, most of them due to heparin’s high sulfate groups. To gain insight into the mechanism of activation of the spectroscopic probe with sulfate groups of heparin in vitro, we have used a cationic dye by a spectrophotometric method. It is considered that th...

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Bibliographic Details
Published in:Talanta (Oxford) 1999-05, Vol.48 (5), p.1095-1101
Main Authors: Jiao, Q.C., Liu, Q., Sun, C., He, H.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Hemoproteins
Heparin
Metalloproteins
Proteins
Spectroscopic probe
Total binding number N
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Summary:Heparin has a variety of biological activities, most of them due to heparin’s high sulfate groups. To gain insight into the mechanism of activation of the spectroscopic probe with sulfate groups of heparin in vitro, we have used a cationic dye by a spectrophotometric method. It is considered that the combination of heparin with methylene blue is due to noncovalent binding forces. Dye binding requires an organic chain structure form with sulfate groups. The solution equilibria of the reaction system are discussed. A new linear regression equation has been proposed, in which the maximum binding number N expresses the binding ability of methylene blue (MB) with sulfate groups of heparin. The linear regression equation can estimate this parameter.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(98)00330-0