Robotic sample pretreatment-immunoassay determination of chlorpyrifos metabolite (TCP) in soil and fruit

A fully automated method for leaching of TCP (3,5,6-trichloro-2-pyridinol, the major degradation product of the widely used chlorpyrifos insecticides) from soil and fruit with subsequent determination by ELISA is reported. The automation of the weighing and leaching steps enables unattended developm...

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Bibliographic Details
Published in:Talanta (Oxford) 1997-12, Vol.45 (2), p.371-377
Main Authors: Velasco-Arjona, A., Manclús, J.J., Montoya, A., Luque de Castro, M.D.
Format: Article
Language:English
Subjects:
3,5,6-Trichloro-2-pyridinol
Agronomy. Soil science and plant productions
Biological and medical sciences
Chlorpyrifos
ELISA
Food industries
Fruit
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Methods and techniques
Robot
Soil
Soil science
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Summary:A fully automated method for leaching of TCP (3,5,6-trichloro-2-pyridinol, the major degradation product of the widely used chlorpyrifos insecticides) from soil and fruit with subsequent determination by ELISA is reported. The automation of the weighing and leaching steps enables unattended development of sample preparation. The determination of the target analyte, traditionally performed by liquid chromatography, has been substituted by specific immunoassay using a non-commercial monoclonal antibody which enables the determination of TCP within the range 0.01−7 ng 1 −1 with an R.S.D. of 8.6%.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(97)00140-9