Micellar versus reversed phase liquid chromatography for the determination of desferrioxamine and its chelates with aluminium and iron in uremic serum

Micellar liquid chromatography with sodium dodecyl sulphate or Brij-35 as surfactants in the mobile phase was evaluated and compared with reversed phase liquid chromatography using conventional acetonitrile-water eluents for the separation and determination of desferrioxamine (DFO) and its complexes...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 1997-12, Vol.45 (1), p.25-33
Main Author: MENENDEZFRAGA, P
Format: Article
Language:English
Subjects:
Aluminoxamine
Analysis
Biological and medical sciences
Desferrioxamine
Ferrioxamine
General pharmacology
Medical sciences
Micellar liquid chromatography
Pharmacology. Drug treatments
Reversed phase liquid chromatography
Uremic serum
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Micellar liquid chromatography with sodium dodecyl sulphate or Brij-35 as surfactants in the mobile phase was evaluated and compared with reversed phase liquid chromatography using conventional acetonitrile-water eluents for the separation and determination of desferrioxamine (DFO) and its complexes with aluminium (AIDFO) and iron (FeDFO) in uremic serum. Reversed phase liquid chromatography proved to be superior in terms of sensitivity and selectivity. The three solutes investigated were separated with a mobile phase of 13% (v/v) acetonitrile/phosphate buffer (5 mM, pH = 3.5) on a C 18 column and detected by ultraviolet absorption at 210 nm (DFO) and 220 nm (AlDFO and FeDFO). Limits of detection of 0.1 μg ml −1 and relative standard deviation of 3–4% were obtained. The recovery from serum samples after ultramicrofiltration was around 90%. The method was applied to the determination of DFO, AlDFO and FeDFO in uremic serum.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(97)00097-0