Biosensor based on paraffin/graphite modified with sweet potato tissue for the determination of hydroquinone in cosmetic cream in organic phase

An organic-phase biosensor based on paraffin/graphite modified with sweet potato ( Ipomoea batatas (L.) Lam.) tissue as the source of peroxidase was developed and used for determining hydroquinone in cosmetic creams. This enzyme in the presence of hydrogen peroxide catalyses the oxidation of hydroqu...

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Bibliographic Details
Published in:Talanta (Oxford) 2000-07, Vol.52 (4), p.681-689
Main Authors: Cruz Vieira, Iolanda, Fatibello-Filho, Orlando
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensor
Biosensors
Biotechnology
Catalyst activity
Chemical modification
Cosmetic cream
Electrochemistry
Enzyme kinetics
Fundamental and applied biological sciences. Psychology
Graphite
Hydrogen peroxide
Hydroquinone
Methods. Procedures. Technologies
Oxidation
Paraffin
Paraffins
Phenols
Reduction
Various methods and equipments
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Summary:An organic-phase biosensor based on paraffin/graphite modified with sweet potato ( Ipomoea batatas (L.) Lam.) tissue as the source of peroxidase was developed and used for determining hydroquinone in cosmetic creams. This enzyme in the presence of hydrogen peroxide catalyses the oxidation of hydroquinone to p-quinone which electrochemical reduction back to hydroquinone was obtained at a peak potential of −0.22 V. The recovery of hydroquinone from two samples ranged from 99.1 to 104.1% and a rectilinear analytical curve for hydroquinone concentration from 7.5×10 −5 to 1.6×10 −3 M ( r=0.9991) were obtained. The detection limit was 8.1×10 −6 M and relative standard deviation was
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(00)00420-3